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椭圆酵母中普通氨基酸通透酶的不同D-葡萄糖需求与蛋白质合成

Differential D-glucose requirements of the general amino-acid permease and protein synthesis in Saccharomyces cerevisiae var. ellipsoideus.

作者信息

Iglesias R, Ferreras J M, Muñoz R, Arias F J, Rojo M A, Girbés T

机构信息

Departamento de Bioquímica y Biología Molecular y Fisiología, Facultad de Ciencias; Universidad de Valladolid, Spain.

出版信息

Cell Mol Biol. 1990;36(5):581-7.

PMID:2073683
Abstract

The dependence of the general aminoacid permease and protein synthesis on the availability of D-glucose as energy source was studied. Stimulation by the sugar was immediate once added to the cell suspensions and seems to be mediated by energy derived directly from glycolysis. The general aminoacid permease was saturated linearly with D-glucose whereas protein synthesis was saturated sigmoidealy requiring much higher concentration of the sugar than the general aminoacid permease.

摘要

研究了一般氨基酸通透酶和蛋白质合成对作为能量来源的D-葡萄糖可用性的依赖性。一旦将糖添加到细胞悬液中,其刺激作用立即显现,并且似乎是由直接来自糖酵解的能量介导的。一般氨基酸通透酶与D-葡萄糖呈线性饱和,而蛋白质合成呈S形饱和,所需糖的浓度比一般氨基酸通透酶高得多。

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