Komissarenko V P, Chelnakova I S, Mikosha A S
Probl Endokrinol (Mosk). 1978 Jan-Feb;24(1):95-8.
A study was made of the inhibitors of the adrenal gland function and ACTH on the activity of NADP-specific glutathionreductase in the adrenal glands and the liver of dogs. After a 10-day feeding to dogs of o,p'-dichlordiphenyldichlorethane (o,p'-DDD) and p,p'-diethyldiphenyl dichlorethane (p,p'-pertane) in a dose of 50 mg/kg there was observed a marked activation of glutathion reductase in the adrenal glands. Elevation of the activity of the enzyme was revealed as soon as 24 hours after the administration of o,p'-DDD. The inhibitors under study failed to influence the activity of the enzyme in the liver of dogs, this indicating a specific action of o,p'-DDD and pertane on the adrenal gland cortex. Stimulation of glucocorticoid biosynthesis under the effect of ACTH administered once in a dose of 25 Units/kg was not accompanied by the changes in the activity of glutathione reductase in the adrenal glands and the liver of dogs. Prolonged ACTH administration (4 Units/kg) caused a significant reduction of the enzyme activity calculated as per 1 mg of protein in the adrenal gland homogenate.
对肾上腺功能抑制剂和促肾上腺皮质激素（ACTH）对犬肾上腺和肝脏中NADP特异性谷胱甘肽还原酶活性的影响进行了研究。给犬喂食剂量为50mg/kg的邻，对'-二氯二苯二氯乙烷（o，p'-DDD）和对，对'-二乙基二苯二氯乙烷（p，p'-培丹）10天后，观察到肾上腺中谷胱甘肽还原酶有明显激活。在给予o，p'-DDD后24小时就发现该酶活性升高。所研究的抑制剂未影响犬肝脏中该酶的活性，这表明o，p'-DDD和培丹对肾上腺皮质有特异性作用。以25单位/kg的剂量一次性给予ACTH，刺激糖皮质激素生物合成，并未伴随犬肾上腺和肝脏中谷胱甘肽还原酶活性的变化。长期给予ACTH（4单位/kg）导致肾上腺匀浆中按每1mg蛋白质计算的酶活性显著降低。
