Stanley Ho Centre for Emerging Infectious Diseases, School of Public Health, The Chinese University of Hong Kong, Shatin, N.T. Hong Kong, China.
J Infect. 2010 Jul;61(2):164-72. doi: 10.1016/j.jinf.2010.04.012. Epub 2010 May 20.
To investigate the molecular epidemiology of recently diagnosed HIV-1 infection in Macao for better understanding the epidemiology in this Chinese city, in context of its relationship with other countries in Asia and the rest of the world.
Serum samples of HIV positive cases reported between 2005 and 2007 were collected from the Macao Public Health Laboratory. HIV genotype was determined by phylogenetic analysis of sequences from gag, RT, and env regions.
A total of 30 HIV positive samples were genotyped. The HIV-1 viruses circulating in Macao were characterized by their relatively high genetic diversity. CRF01_AE was predominant (56%), followed by subtype B (13%), CRF12_BF (10%), G/CRF12_BF, A1/CRF10_AD and CRF07_BC, of which CRF12_BF and G/CRF12_BF were first reported in Southeast Asia. Phylogenetic analysis showed that there was no clear clustering of CRF01_AE strains but a distinct CRF12_BF cluster associated with injection drug use could be delineated.
The results suggested that there were multiple introductions of HIV strains in Macao that have been circulating for an extended period of time, superimposed by an outbreak in injection drug users.
研究澳门新诊断的 HIV-1 感染的分子流行病学情况，以便更好地了解这个中国城市的流行病学情况，并结合其与亚洲其他国家和世界其他地区的关系。
从澳门公共卫生化验所收集了 2005 年至 2007 年间报告的 HIV 阳性病例的血清样本。通过对 gag、RT 和 env 区序列的系统进化分析来确定 HIV 基因型。
共对 30 份 HIV 阳性样本进行了基因分型。澳门流行的 HIV-1 病毒具有相对较高的遗传多样性。CRF01_AE 占主导地位（56%），其次是亚型 B（13%）、CRF12_BF（10%）、G/CRF12_BF、A1/CRF10_AD 和 CRF07_BC，其中 CRF12_BF 和 G/CRF12_BF 是首次在东南亚报道的。系统进化分析表明，CRF01_AE 株没有明显的聚类，但可以划出一个与注射吸毒有关的独特的 CRF12_BF 聚类。
结果表明，澳门存在多种 HIV 株的传入，且已经长时间流行，同时还伴有注射吸毒者中的暴发。
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