Liu Shengya, Xing Hui, He Xiang, Xin Ruolei, Zhang Yuanzhi, Zhu Jiahong, Shao Yiming
State Key Laboratory for Infectious Diseases Prevention and Control, National Center for AIDS/STD Control and Prevention, Beijing, China.
Arch Virol. 2008;153(7):1233-40. doi: 10.1007/s00705-008-0107-9. Epub 2008 May 27.
The aim of this study was to investigate the genetic variation of HIV-1 CRF07_BC, the most prevalent circulating strain in intravenous drug users (IDUs) in China. We studied the diversity in the C2-V5 region of the HIV-1 env gene and in the p17-p24 region of the HIV-1 gag gene from the same samples in 12 IDUs who were divided into two groups according to the length of infection time. Two IDUs were longitudinally monitored from the time of seroconversion for 2-2.5 years. The viral divergence from the founder strain and the viral population diversity between sequential time points were analyzed in two men. The data show that the divergence of the env gene is higher than that of gag in general, while the diversity of the gag gene is sometimes higher than that of env during the course of HIV evolution. In addition, env and gag gene diversity increased over time. The observed patterns and associations may enhance our understanding of HIV-1 evolution.
本研究的目的是调查中国静脉吸毒者中最流行的传播毒株HIV-1 CRF07_BC的基因变异情况。我们研究了12名静脉吸毒者同一样本中HIV-1 env基因的C2-V5区域和HIV-1 gag基因的p17-p24区域的多样性，这些吸毒者根据感染时间长短分为两组。两名吸毒者从血清转化时开始进行了2至2.5年的纵向监测。对两名男性分析了与奠基毒株的病毒差异以及连续时间点之间的病毒群体多样性。数据表明，总体而言，env基因的差异高于gag基因，而在HIV进化过程中，gag基因的多样性有时高于env基因。此外，env和gag基因多样性随时间增加。观察到的模式和关联可能会增进我们对HIV-1进化的理解。
