激光光解系统消融晶状体上皮细胞：组织病理学、超微结构和免疫化学。
Ablation of lens epithelial cells with a laser photolysis system: histopathology, ultrastructure, and immunochemistry.
机构信息
John A. Moran Eye Center, University of Utah, Salt Lake City, Utah 84132, USA.
出版信息
J Cataract Refract Surg. 2010 Jun;36(6):1003-10. doi: 10.1016/j.jcrs.2009.11.021.
PURPOSE
To evaluate efficacy of a neodymium:YAG (Nd:YAG) laser photolysis system in removing lens epithelial cells (LECs) and characterize the effect of the laser on laminin and fibronectin involved in LEC adhesion and migration.
METHODS
Cadaver eyes were evaluated using the Miyake technique. The lenses were removed with phacoemulsification. The modified Nd:YAG laser was used to clean the LECs from the capsule. Only the fornix was cleaned in some eyes and the anterior subcapsular area in other eyes. Some areas were not treated and acted as controls. Standard irrigation/aspiration (I/A) removal of LECs was performed in additional eyes. The eyes were analyzed using light microscopy and immunohistochemical staining.
RESULTS
Histopathologic evaluation showed that the laser removed the LECs from the anterior lens capsule and from the fornix. Immunohistochemical staining showed fibronectin and laminin staining in the untreated areas that was absent in the treated areas. Standard I/A removal of the LECs showed absence of cells but persistent laminin and fibronectin. Electron microscopy showed epithelial cells in untreated areas with an absence of the LECs and debris in treated areas.
CONCLUSIONS
The laser photolysis system removed LECs from the anterior lens capsule and capsule fornix. Along with the cells, laminin, fibronectin, and cell debris remained in the untreated areas but were removed by the treatment. This treatment may be useful in preventing posterior capsule opacification.
目的
评估钕：钇铝石榴石（Nd：YAG）激光光解法系统去除晶状体上皮细胞（LEC）的疗效，并研究激光对层粘连蛋白和纤维连接蛋白的影响，这两种蛋白与 LEC 黏附和迁移有关。
方法
使用 Miyake 技术对尸体眼球进行评估。采用超声乳化法去除晶状体。改良后的 Nd：YAG 激光用于从囊袋中清除 LEC。一些眼球仅从前房角清理，另一些则从前囊下区清理。有些区域未作处理，作为对照。在另外一些眼球中进行标准的冲洗/抽吸（I/A）清除 LEC。使用光镜和免疫组织化学染色对眼球进行分析。
结果
组织病理学评估显示，激光可从前晶状体囊和前房角清除 LEC。免疫组织化学染色显示，未处理区域存在纤维连接蛋白和层粘连蛋白染色，而处理区域则不存在。标准的 I/A 清除 LEC 显示细胞缺失，但层粘连蛋白和纤维连接蛋白仍然存在。电子显微镜显示，未处理区域的上皮细胞存在，但缺乏 LEC 和细胞碎片，而处理区域则没有细胞。
结论
激光光解法系统可从前晶状体囊和囊袋前房角清除 LEC。与细胞一起，未处理区域的层粘连蛋白、纤维连接蛋白和细胞碎片仍然存在，但通过治疗被清除。这种治疗方法可能有助于预防后发性白内障。
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