罗波 7-O-D-葡萄糖苷对人鼻病毒复制的抑制作用。

Inhibitory effects of orobol 7-O-D-glucoside from banaba (Lagerstroemia speciosa L.) on human rhinoviruses replication.

机构信息

Daejeon Health Sciences Colllege, Dong-Gu, Daejeon, South Korea.

出版信息

Lett Appl Microbiol. 2010 Jul;51(1):1-5. doi: 10.1111/j.1472-765X.2010.02845.x. Epub 2010 Mar 29.

DOI:10.1111/j.1472-765X.2010.02845.x

PMID:20497313

Abstract

AIMS

The anti-human rhinovirus (HRV) activity of orobol 7-O-d-glucoside (O7G) from Lagerstroemia speciosa L. (Lythraceae) was evaluated in Hela cells.

METHODS AND RESULTS

We tested anti-HRV activity of O7G using a cytopathic effect (CPE) reduction method, which exhibited broad-spectrum anti-HRVs activity with a 50% inhibitory concentration (IC(50)) ranging from 0.58 to 8.80 microg ml(-1). The 50% cytotoxicity concentration (CC(50)) of O7G is more than 100 microg ml(-1), and the derived therapeutic indices are more than 12. Ribavirin didn't possess antiviral activity against HRV15, HRV3 and HRV5, but exhibited weak antiviral activity against HRV2 and HRV3, and showed strong anti-HRV6 and -14 activities.

CONCLUSIONS

These results suggest that O7G is a novel drug class with broad spectrum antiviral activity against HRV species A (HRV1B, HRV2, HRV15 and HRV40) and species B (HRV3, HRV6 and HRV14), as well as pleconaril-resistant virus (HRV5).

SIGNIFICANCE AND IMPACT OF THE STUDY

Therefore, these findings provide important information for the utilization of Q7G promising broad spectrum for human rhinovirus treatment.

摘要

目的

评估千屈菜科紫薇（ Lagerstroemia speciosa L.）中的 7-O-β-D-葡萄糖基紫堇醇灵（O7G）抗人鼻病毒（HRV）的活性。

方法和结果

我们使用细胞病变效应（CPE）减少法测试了 O7G 的抗 HRV 活性，结果显示其具有广谱抗 HRV 活性，半数抑制浓度（IC50）范围为 0.58 至 8.80 μg/ml。O7G 的 50%细胞毒性浓度（CC50）大于 100 μg/ml，其治疗指数大于 12。利巴韦林对 HRV15、HRV3 和 HRV5 没有抗病毒活性，但对 HRV2 和 HRV3 表现出较弱的抗病毒活性，对 HRV6 和 HRV14 表现出较强的抗病毒活性。