UV 光和乙醇浸泡处理后，克罗诺杆菌生物膜在硅树脂、聚碳酸酯和不锈钢上的成熟和存活。

Maturation and survival of Cronobacter biofilms on silicone, polycarbonate, and stainless steel after UV light and ethanol immersion treatments.

机构信息

Department of Food Science and Technology, Chung-Ang University, 72-1 Naeri, Ansung, Gyunggi-do 456-756, South Korea.

出版信息

J Food Prot. 2010 May;73(5):952-6. doi: 10.4315/0362-028x-73.5.952.

DOI:10.4315/0362-028x-73.5.952

PMID:20501047

Abstract

Cronobacter sakazakii cells in biofilms formed on silicone, polycarbonate, and stainless steel coupons immersed in reconstituted powdered infant milk formula were treated with ethanol (10 to 70%) and UV light (12 to 2,160 mW.s/cm(2)) as antibacterial treatments. Biofilm maturation curves were determined after immersion at 25 degrees C for up to 144 h. Populations increased after subsequent immersion at 25 degrees C for 24 h in reconstituted powdered infant milk formula to the respective maximum levels of 7.96, 7.91, and 6.99 log CFU per coupon. Populations attached to silicone and polycarbonate surfaces to a greater extent than to stainless steel (P < 0.05). Treatment with 10% ethanol did not cause a significant decrease in the level of C. sakazakii, but treatment with 30, 40, and 50% ethanol reduced the levels to approximately 1.73, 3.02, and 4.17 log CFU per coupon, respectively. C. sakazakii was not detected on any coupon after treatment with 70% ethanol or 2,160 mW.s/cm(2) UV light. A synergistic effect of sequential ethanol and UV treatments was not observed.

摘要

在 25°C 下浸泡长达 144 小时后，确定生物膜成熟曲线。在随后的 25°C 下，将再水合的婴儿配方粉奶粉浸泡 24 小时后，生物膜中 Cronobacter sakazakii 细胞的数量增加到每个样品卡的 7.96、7.91 和 6.99 log CFU 的相应最高水平。与不锈钢相比，硅胶和聚碳酸酯表面的细菌数量增加得更多（P<0.05）。用 10%乙醇处理不会导致 C.sakazakii 的水平显著下降，但用 30%、40%和 50%乙醇处理会使水平分别降低至每个样品卡约 1.73、3.02 和 4.17 log CFU。在用 70%乙醇或 2,160 mW.s/cm²UV 光处理后，在任何样品卡上都未检测到 C.sakazakii。未观察到乙醇和 UV 处理顺序的协同作用。