反式肉桂醛对无生命表面阪崎克罗诺杆菌生物膜的抑制和失活动力学的影响。

Effect of trans-cinnamaldehyde on inhibition and inactivation of Cronobacter sakazakii biofilm on abiotic surfaces.

机构信息

Department of Animal Science, University of Connecticut, Storrs, Connecticut 06269, USA.

出版信息

J Food Prot. 2011 Feb;74(2):200-8. doi: 10.4315/0362-028X.JFP-10-296.

Abstract

The efficacy of trans-cinnamaldehyde (TC), an ingredient in cinnamon oil, for inhibiting biofilm synthesis (560 and 750 μM TC) and inactivating mature biofilms (23 and 38 mM TC) of Cronobacter sakazakii was investigated at 24 and 12 °C in the presence and absence of reconstituted infant formula on polystyrene plates, stainless steel coupons, feeding bottle coupons, and enteral feeding tube coupons. Additionally, TC's effect on the expression of genes critical for biofilm formation in C. sakazakii was determined by reverse transcription quantitative PCR. TC inhibited and inactivated C. sakazakii biofilms on all matrices tested at both temperatures. C. sakazakii was reduced by >4.0 and 3.0 log CFU/ml after 96 h of exposure to 38 mM and 750 μM TC, respectively. Reverse transcription quantitative PCR results revealed that TC significantly (P≤0.05) down-regulated biofilm-associated genes in C. sakazakii. TC could potentially be used to control C. sakazakii biofilms on infant formula feeding equipment and preparatory areas.

摘要

研究了肉桂醛（TC）作为肉桂油的一种成分，在 24℃和 12℃条件下，有无婴儿配方奶粉复水的情况下，在聚苯乙烯板、不锈钢试片、奶瓶试片和肠内喂养管试片上对阪崎克罗诺杆菌生物膜合成（560 和 750μM TC）和成熟生物膜（23 和 38mM TC）的抑制和灭活作用。此外，通过反转录定量 PCR 确定了 TC 对阪崎克罗诺杆菌生物膜形成关键基因表达的影响。TC 在所有测试基质上均能抑制和灭活在两种温度下的 C. sakazakii 生物膜。暴露于 38mM 和 750μM TC 96 小时后，C. sakazakii 的减少量分别超过 4.0 和 3.0 log CFU/ml。反转录定量 PCR 结果表明，TC 显著（P≤0.05）下调了 C. sakazakii 生物膜相关基因。TC 可能有潜力用于控制婴儿配方奶粉喂养设备和准备区域的 C. sakazakii 生物膜。