Song Hana, Lee Sun-Young
Department of Food and Nutrition, Chung-Ang University, 4726, Seodong-daero, Anseong-si, Gyeonggi-do Republic of Korea.
Food Sci Biotechnol. 2020 May 27;29(9):1241-1250. doi: 10.1007/s10068-020-00773-z. eCollection 2020 Sep.
This study investigated the survivals of two pathogens ( O157:H7 and ) in different adhered forms on glass fiber filters (GFFs) at 43 and 68% relative humidity (RH). Efficacies of chemical sanitizers at reducing pathogenic biofilms on GFFs were also evaluated. Inoculated GFFs were incubated at 28 °C in TSB (type I), on TSA (type II), or on TSA under 100% RH (type III) to produce biofilms. When GFFs were incubated at 43 or 68% RH for 7 days, type III biofilms were less than 2 log CFU/filter reduction whereas type I and type II biofilms were 4-6 log CFU/filter reduction. Additionally, type III biofilms were highly resistant to sanitizing treatment compared than other biofilms (type I and II). Therefore, the method to produce biofilms used in this study could be used to produce highly resistance pathogenic biofilms in the laboratory for related experiments.
本研究调查了两种病原体（O157:H7和）在43%和68%相对湿度（RH）下以不同附着形式存在于玻璃纤维滤器（GFF）上的存活情况。还评估了化学消毒剂减少GFF上致病生物膜的效果。将接种后的GFF在28°C下于胰酪大豆肉汤（I型）中、在胰酪大豆琼脂（II型）上或在100%RH条件下的胰酪大豆琼脂上（III型）培养以形成生物膜。当GFF在43%或68%RH下培养7天时，III型生物膜的减少量小于2 log CFU/滤器，而I型和II型生物膜的减少量为4 - 6 log CFU/滤器。此外，与其他生物膜（I型和II型）相比，III型生物膜对消毒处理具有高度抗性。因此，本研究中用于产生生物膜的方法可用于在实验室中产生高度抗性的致病生物膜以进行相关实验。
