Cardone Maria Francesca, Ventura Mario, Tempesta Sergio, Rocchi Mariano, Archidiacono Nicoletta
Sezione di Genetica, DAPEG, Via Amendola 165/A, 70126 Bari, Italy.
Chromosoma. 2002 Dec;111(5):348-56. doi: 10.1007/s00412-002-0215-3. Epub 2002 Oct 9.
A panel of human chromosome painting probes and bacterial and P1 artificial chromosome (BAC/PAC) clones were used in fluorescence in situ hybridization (FISH) experiments to investigate the chromosome conservation of the ring-tailed lemur (Lemur catta, LCA) with respect to human. Whole chromosome paints specific for human chromosomes 7, 9, 11, 13, 14, 17, 18, 20, 21, and X were found to identify a single chromosome or an uninterrupted chromosomal region in LCA. A large set of partial chromosome paints and BAC/PAC probes were then used to refine the characterization of the rearrangements differentiating the two karyotypes. The results were also used to reconstruct the ancestral Lemuridae karyotype. Lemur catta, indeed, can be used as an outgroup, allowing symplesiomorphic (ancestral) rearrangements to be distinguished from apomorphic (derived) rearrangements in lemurs. Some LCA chromosomes are difficult to distinguish morphologically. The 'anchorage' of most LCA chromosomes to specific probes will contribute to the standardization of the karyotype of this species.
一组人类染色体涂染探针以及细菌和P1人工染色体（BAC/PAC）克隆被用于荧光原位杂交（FISH）实验，以研究环尾狐猴（Lemur catta，LCA）相对于人类的染色体保守性。发现针对人类染色体7、9、11、13、14、17、18、20、21和X的全染色体涂染能够识别LCA中的一条单一染色体或一个不间断的染色体区域。然后使用大量部分染色体涂染和BAC/PAC探针来细化区分两种核型的重排特征。这些结果还被用于重建狐猴科祖先核型。事实上，环尾狐猴可以用作外类群，从而能够区分狐猴中的同形（祖先）重排和异形（衍生）重排。一些LCA染色体在形态上难以区分。大多数LCA染色体与特定探针的“锚定”将有助于该物种核型的标准化。
