Etienne J, Charpin B, Grando J, Brun Y, Bes M, Fleurette J
Centre National de Référence des Staphylocoques, Faculté de Médecine Alexis Carrel, Lyon, France.
Epidemiol Infect. 1991 Jun;106(3):467-75. doi: 10.1017/s0950268800067510.
Biotyping, slime production, antibiograms, extrachromosomal DNA banding and total DNA restriction analysis were used to characterize Staphylococcus epidermidis strains causing cerebrospinal fluid shunt infections in 11 patients. Infections considered to be community acquired and those acquired in the first 2 weeks of hospital admission were due to oxacillin-susceptible isolates. Multiply resistant strains were isolated from patients who were in hospital for more than 1 month before tube implantation. Slime was detected in staphylococci for 54% of cases, but its expression varied. Strains from different patients could be differentiated from one another by the extrachromosomal DNA bandings and total DNA restriction patterns, but isolates from the same patient were usually similar. During the period of external drainage, epidemiological markers were useful in differentiating persistence of infection from contamination or re-infection by a new strain.
采用生物分型、黏液产生情况、抗菌谱、染色体外DNA条带分析及总DNA限制性分析，对11例引起脑脊液分流感染的表皮葡萄球菌菌株进行特征分析。被认为是社区获得性感染以及入院后前2周内获得的感染是由对苯唑西林敏感的分离株引起的。多重耐药菌株是从置管前住院超过1个月的患者中分离出来的。54%的病例中葡萄球菌检测到黏液，但黏液表达情况各异。不同患者的菌株可通过染色体外DNA条带和总DNA限制性图谱相互区分，但同一患者的分离株通常相似。在外引流期间，流行病学标志物有助于区分感染持续存在与污染或新菌株再感染。
