Department of Genetics, Development and Cell Biology, Iowa State University, Ames, Iowa 50011, USA.
Dev Dyn. 2010 Jun;239(6):1849-57. doi: 10.1002/dvdy.22307.
The CD133/prominin1 gene encodes a pentamembrane glycoprotein cell surface marker that is expressed in stem cells from neuroepithelial, hematopoietic, and various organ tissues. Here we report the analysis of two zebrafish CD133/prominin1 orthologues, prominin1a and prominin1b. The expression patterns of the zebrafish prominin1a and b genes were analyzed during embryogenesis using whole mount in situ hybridization. prominin1a and b show novel complementary and overlapping patterns of expression in proliferating zones in the developing sensory organs and central nervous system. The expression patterns suggest functional conservation of the zebrafish prominin1 genes. Initial analyses of prominin1a and b in neoplastic tissue show increased expression of both genes in a subpopulation of cells in malignant peripheral nerve sheath tumors in tp53 mutants. Based on these analyses, the zebrafish prominin1 genes will be useful markers for examining proliferating cell populations in adult organs, tissues, and tumors.
CD133/prominin1 基因编码一种五跨膜糖蛋白细胞表面标志物，在神经上皮、造血和各种器官组织的干细胞中表达。在这里，我们报告了对两种斑马鱼 CD133/prominin1 同源物 prominin1a 和 prominin1b 的分析。使用整体原位杂交技术在胚胎发生过程中分析了斑马鱼 prominin1a 和 b 基因的表达模式。prominin1a 和 b 在发育中的感觉器官和中枢神经系统的增殖区表现出新颖的互补和重叠表达模式。这些表达模式表明斑马鱼 prominin1 基因具有功能保守性。在肿瘤组织中对 prominin1a 和 b 的初步分析表明，在 tp53 突变体的恶性外周神经鞘瘤中，这两个基因在亚群细胞中的表达增加。基于这些分析，斑马鱼 prominin1 基因将成为研究成年器官、组织和肿瘤中增殖细胞群体的有用标志物。
