耐盐真菌 Aspergillus sclerotiorum PT06-1 中的环三肽。

Cyclic tripeptides from the halotolerant fungus Aspergillus sclerotiorum PT06-1.

机构信息

Key Laboratory of Marine Drugs, Chinese Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, People's Republic of China.

出版信息

J Nat Prod. 2010 Jun 25;73(6):1133-7. doi: 10.1021/np100198h.

DOI:10.1021/np100198h

PMID:20503985

Abstract

Eleven new aspochracin-type cyclic tripeptides, sclerotiotides A-K (1-11), together with three known compounds, JBIR-15 (12), aspochracin (13), and penicillic acid, were isolated from the ethyl acetate extract of the fermentation broth of the halotolerant Aspergillus sclerotiorum PT06-1 in a hypersaline nutrient-rich medium. Their structures were elucidated by spectroscopic analysis and chemical methods. Chemical transformations of 12 and 13 proved that sclerotiotides D-K (4-11) were artifacts probably formed during the fermentation or subsequent isolation steps. All 13 cyclic tripeptides have been evaluated for their antimicrobial and cytotoxic effects. Only sclerotiotides A (1), B (2), F (6), and I (9) and JBIR-15 (12) showed selective antifungal activity against Candida albicans with MIC values of 7.5, 3.8, 30, 6.7, and 30 microM, respectively.

摘要

从耐盐营养丰富培养基中盐生曲霉 PT06-1 的发酵液的乙酸乙酯提取物中分离到 11 种新的 Asperchracin 型环三肽，即 sclerotiotides A-K（1-11），以及三种已知化合物，JBIR-15（12），asperchracin（13）和青霉素酸。通过光谱分析和化学方法阐明了它们的结构。12 和 13 的化学转化证明 sclerotiotides D-K（4-11）可能是在发酵或随后的分离步骤中形成的。所有 13 种环三肽都进行了抗微生物和细胞毒性评估。只有 sclerotiotides A（1），B（2），F（6）和 I（9）和 JBIR-15（12）对白色念珠菌表现出选择性抗真菌活性，MIC 值分别为 7.5、3.8、30、6.7 和 30 microM。