一种测定生物组织中牛磺酸的快速方法。

A rapid method for the determination of taurine in biological tissue.

作者信息

Hopkins P C, Kay I S, Davies W E

机构信息

Department of Pharmacology, Medical School, University of Birmingham, Birmingham B15 2TJ, England.

出版信息

Neurochem Int. 1989;15(4):429-32. doi: 10.1016/0197-0186(89)90160-5.

DOI:10.1016/0197-0186(89)90160-5

PMID:20504516

Abstract

A rapid method for the determination of taurine is described. Based on high performance liquid chromatography (HPLC) separation, it avoids all transfers, precleaning treatments and the use of internal standards. The method consists of precolumn derivatization with O-phthaldialdehyde (OPA) followed by separation on a reversed phase styrene column using an acetonitrile-citrate buffer mixture as the eluting solvent. Naturally occurring substances, often quoted to co-migrate with taurine, are separated and the method described has been used to determine the taurine content of various tissues and blood fractions. These determinations indicate that the only satisfactory determination of blood taurine is that of whole blood.

摘要

本文描述了一种快速测定牛磺酸的方法。该方法基于高效液相色谱（HPLC）分离，避免了所有转移、预清洗处理以及内标物的使用。该方法包括用邻苯二甲醛（OPA）进行柱前衍生，然后在反相苯乙烯柱上进行分离，使用乙腈 - 柠檬酸盐缓冲液混合物作为洗脱溶剂。通常被认为与牛磺酸共迁移的天然物质得以分离，并且所描述的方法已用于测定各种组织和血液组分中的牛磺酸含量。这些测定结果表明，血液中牛磺酸唯一令人满意的测定是全血的测定。