Bhatnagar M, Oka H, Ito Y
Laboratory of Technical Development, National Heart, Lung, and Blood Institute, Bethesda, MD 20892.
J Chromatogr. 1989 Feb 3;463(2):317-28. doi: 10.1016/s0021-9673(01)84486-1.
Performance of the apparatus was evaluated in terms of stationary phase retention, partition efficiency and sample loading capacity. Preliminary studies with short coils revealed high retention of the stationary phase under a proper combination of the head-tail elution and planetary motion. Preparative capability of the apparatus was successfully demonstrated on efficient multigram separations of 2,4-dinitrophenyl amino acids, indole auxins, and bacitracin in a pair of large multilayer coils with a total capacity of 1.5 l.
根据固定相保留、分配效率和进样量对该仪器的性能进行了评估。对短线圈的初步研究表明，在头-尾洗脱和行星运动的适当组合下，固定相具有高保留率。该仪器的制备能力已通过在一对总容量为1.5升的大型多层线圈中对2,4-二硝基苯基氨基酸、吲哚生长素和杆菌肽进行高效的数克级分离而得到成功证明。
