Zhang Gui-yun, Chen Zuo-quan, Ling Feng, Li Yu-jian, Wang Yan, Gu Bin-xian
Department of Neurosurgery, Tongji Hospital of Tongji University, Shanghai-200 065, China.
Neurol India. 2010 Mar-Apr;58(2):191-4. doi: 10.4103/0028-3886.63782.
Atherosclerosis is the most common cause of ischemic stroke. Until now, there has been no ideal animal model for studying the hemodynamic ischemia caused by atherosclerosis in posterior circulation.
This study was conducted to establish a cerebral ischemic model in an atherosclerotic rabbit by subclavian artery (SA) ligation or embolization, emphasizing the role of hemodynamics.
Thirty-two male New Zealand rabbits (weight, 3.5 to 4.0 kg; provided by Animal Laboratory of Tongji Hospital of Tongji University) were randomly divided into four groups. All the groups, except the controlled group, were fed with high lipid diet for three months and two of the groups were submitted for right SA ligation or left SA embolization and the remaining one group to non-operated group.
A SA steal syndrome was established successfully with delayed visualization and prolonged circulation time in the posterior circulation. The circulation time was different when SA was ligated or embolized (P < 0.001), and also there was statistically significant difference between controlled and non-operated groups when angiography of either, left and right, VA was performed (P = 0.009, P = 0.003, respectively). However, there was no statistical significant difference between the ligation and embolization group either before or after the procedure (ligation or embolization) (P = 0.402, P = 0.068, respectively). In the control and non-operated group, angiography of either left or right VA showed no statistically significant difference (P = 0.285, P = 0.197, respectively). In the groups fed with high lipid diet for three months, atherosclerotic plaques were found in the common carotid arteries (CCAs), aortic arch (AAs) and variably in the internal carotid arteries (ICAs).
This model could, to some extent, simulate the clinical hemodynamic state of atherosclerotic stroke. and it is an ideal model for studying the hemodynamic cerebral ischemia in posterior circulation.
动脉粥样硬化是缺血性中风最常见的病因。到目前为止，尚无理想的动物模型用于研究后循环中由动脉粥样硬化引起的血流动力学缺血。
本研究旨在通过结扎或栓塞锁骨下动脉（SA）在动脉粥样硬化兔中建立脑缺血模型，重点研究血流动力学的作用。
32只雄性新西兰兔（体重3.5至4.0千克；由同济大学附属同济医院动物实验室提供）随机分为四组。除对照组外，所有组均给予高脂饮食三个月，其中两组进行右侧SA结扎或左侧SA栓塞，另一组为非手术组。
成功建立了SA盗血综合征，后循环出现延迟显影和循环时间延长。SA结扎或栓塞时循环时间不同（P < 0.001），左右椎动脉血管造影时，对照组与非手术组之间也存在统计学显著差异（分别为P = 0.009，P = 0.003）。然而，手术（结扎或栓塞）前后结扎组和栓塞组之间无统计学显著差异（分别为P = 0.402，P = 0.068）。在对照组和非手术组中，左右椎动脉血管造影均无统计学显著差异（分别为P = 0.285，P = 0.197）。在给予高脂饮食三个月的组中，在颈总动脉（CCA）、主动脉弓（AA）以及颈内动脉（ICA）中均发现动脉粥样硬化斑块。
该模型在一定程度上可以模拟动脉粥样硬化性中风的临床血流动力学状态，是研究后循环血流动力学性脑缺血的理想模型。
