Endocrinology and Metabolism Research Center (EMRC), Vali-Asr Hospital, Tehran University of Medical Sciences, Tehran, Iran.
Department of Biomedicine, Neurosciences and Advanced Diagnostics, University of Palermo, Palermo, Italy.
Oxid Med Cell Longev. 2021 Jul 31;2021:8864905. doi: 10.1155/2021/8864905. eCollection 2021.
Colorectal cancer (CRC) as one the most common cancer type is associated with oxidative stress. Surgery is the only curative modality for early-stage CRC. The aim of this study was to evaluate the oxidative damage biomarkers as well as enzymatic and nonenzymatic antioxidants in patients with CRC before and after tumor resection and in healthy controls. 60 patients with stage I/II colorectal adenocarcinoma and 43 healthy controls were recruited in this study. We measured plasma levels of oxidative damage biomarkers, including advanced oxidation protein products (AOPP), advanced glycation end products (AGEs), malondialdehyde (MDA), and oxidized low-density lipoprotein (ox-LDL) at baseline and after tumor removal. We also evaluated the plasma activity of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) as enzymatic antioxidants and the ferric reducing antioxidant power (FRAP) assay for nonenzymatic antioxidant capacity. Patients with CRC had significantly higher AGE, AOPP, MDA, and ox-LDL and also FRAP levels and higher SOD and GPx and lower CAT activity levels compared to healthy controls ( < 0.05). We did not observe any statistically significant correlation between redox biomarkers and the size and stage of the tumor. AGEs (72.49 ± 4.7 vs. 67.93 ± 8.8, < 0.001), AOPP (137.64 ± 21.9 vs. 119.08 ± 33.1, < 0.001), MDA (3.56 ± 0.30 vs. 3.05 ± 0.33, < 0.001), and ox-LDL (19.78 ± 0.97 vs. 16.94 ± 1.02, < 0.001) concentrations reduced significantly after tumor removal. The largest effect sizes were found in ox-LDL ( = -2.853, 95% CI 2.50-3.19) and MDA ( = -1.617, 95% CI 0.43-0.57). Serum FRAP levels (1097.5 ± 156.7 vs. 1239.3 ± 290, < 0.001) and CAT (2.34 ± 0.34 vs. 2.63 ± 0.38, < 0.001), GPx (102.37 ± 6.58 vs. 108.03 ± 6.95, < 0.001), and SOD (5.13 ± 0.39 vs. 5.53 ± 0.31, < 0.001) activity levels increased significantly after surgery. The largest effect sizes among antioxidants were seen in SOD ( = 1.135, 95% CI 0.46-0.34) and GPx ( = 0.836, 95% CI 0.35-0.23). This study indicated that patients with colorectal cancer had higher levels of oxidative stress and antioxidant activity compared to healthy controls. After surgical resection of tumor, we observed a substantial improvement in redox homeostasis.
结直肠癌（CRC）是最常见的癌症类型之一，与氧化应激有关。手术是早期 CRC 的唯一治愈方式。本研究旨在评估 CRC 患者肿瘤切除前后及健康对照者的氧化损伤生物标志物以及酶和非酶抗氧化剂。本研究纳入了 60 例 I/II 期结直肠腺癌患者和 43 名健康对照者。我们在基线和肿瘤切除后测量了氧化损伤生物标志物的血浆水平，包括晚期氧化蛋白产物（AOPP）、晚期糖基化终产物（AGE）、丙二醛（MDA）和氧化型低密度脂蛋白（ox-LDL）。我们还评估了超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GPx）的血浆活性作为酶抗氧化剂，以及铁还原抗氧化能力（FRAP）测定非酶抗氧化能力。与健康对照组相比，CRC 患者的 AGE、AOPP、MDA 和 ox-LDL 以及 FRAP 水平更高，SOD 和 GPx 活性更高，CAT 活性更低（<0.05）。我们没有观察到氧化还原生物标志物与肿瘤的大小和分期之间存在任何统计学显著相关性。AGE（72.49±4.7 vs. 67.93±8.8，<0.001）、AOPP（137.64±21.9 vs. 119.08±33.1，<0.001）、MDA（3.56±0.30 vs. 3.05±0.33，<0.001）和 ox-LDL（19.78±0.97 vs. 16.94±1.02，<0.001）浓度在肿瘤切除后显著降低。ox-LDL（=-2.853，95%CI 2.50-3.19）和 MDA（=-1.617，95%CI 0.43-0.57）的效应量最大。血清 FRAP 水平（1097.5±156.7 vs. 1239.3±290，<0.001）和 CAT（2.34±0.34 vs. 2.63±0.38，<0.001）、GPx（102.37±6.58 vs. 108.03±6.95，<0.001）和 SOD（5.13±0.39 vs. 5.53±0.31，<0.001）活性水平在手术后显著升高。抗氧化剂中最大的效应量出现在 SOD（=1.135，95%CI 0.46-0.34）和 GPx（=0.836，95%CI 0.35-0.23）。本研究表明，与健康对照组相比，结直肠癌患者的氧化应激和抗氧化活性水平更高。在肿瘤切除后，我们观察到氧化还原平衡有了显著改善。
