Department of Medical Biochemistry, Faculty of Medicine, Tanta University, Tanta 31111, Egypt.
World J Gastroenterol. 2011 May 21;17(19):2417-23. doi: 10.3748/wjg.v17.i19.2417.
To investigate the role of p53 antibodies (p53Abs), metallothioneins (MTs) and oxidative stress markers in the early detection of dysplasia in chronic ulcerative colitis (UC).
The study included 30 UC patients, 15 without dysplasia (group II) and 15 with dysplasia (group III), in addition to 15 healthy volunteers (group I, control subjects). The enzyme-linked immunosorbent assay technique was used to measure serum p53Abs and MTs, while advanced oxidation protein products (AOPPs), and reduced glutathione (GSH) levels were measured by spectrophotometric method in all subjects.
In group II and group III compared to group I, there were significant increases in serum levels of AOPPs (145.94 ± 29.86 μmol/L and 192.21 ± 46.71 μmol/L vs 128.95 ± 3.06 μmol/L, P < 0.002 and P < 0.001, respectively), MTs (8.18 ± 0.35 μg/mL and 9.20 ± 0.58 μg/mL vs 6.12 ± 0.25 μg/mL, P < 0.05 and P < 0.05, respectively), and p53Abs (20.19 ± 3.20 U/mL and 34.66 ± 1.34 U/mL vs 9.42 ± 1.64 U/mL, P < 0.001 and P < 0.001, respectively). There were significantly higher levels of AOPPs (P < 0.05) and p53Abs (P < 0.001) in UC patients with dysplasia compared to those without dysplasia, while MTs showed no significant difference between the 2 groups (P > 0.096). In contrast, GSH levels showed a significant decrease in both patients' groups (1.87 ± 0.02 μmol/mL and 1.37 ± 0.09 μmol/mL vs 2.49 ± 0.10 μmol/mL, P < 0.05 and P < 0.05 in groups II and III, respectively) compared with group I, and the levels were significantly lower in group III than group II (P < 0.05). There was a positive correlation between AOPPs and both MTs (r = 0.678, P < 0.001) and p53Abs (r = 0.547, P < 0.001), and also between p53Abs and MTs (r = 0.739, P < 0.001). There was a negative correlation between AOPPs and GSH (r = -0.385, P < 0.001), and also between GSH and both MTs (r = -0.662, P < 0.001) and p53Abs (r = -0.923, P < 0.001).
Oxidative stress and oxidative cellular damage play an important role in the pathogenesis of chronic UC and the associated carcinogenetic process. p53Abs levels could help in early detection of dysplasia in these conditions.
研究 p53 抗体（p53Abs）、金属硫蛋白（MTs）和氧化应激标志物在慢性溃疡性结肠炎（UC）异型增生早期检测中的作用。
研究纳入 30 例 UC 患者，其中无异型增生组（II 组）15 例，异型增生组（III 组）15 例，另选 15 例健康志愿者作为对照组（I 组）。采用酶联免疫吸附试验技术检测血清 p53Abs 和 MTs，分光光度法检测各组受试者血清中晚期氧化蛋白产物（AOPPs）和还原型谷胱甘肽（GSH）水平。
与 I 组相比，II 组和 III 组患者血清 AOPPs 水平（145.94 ± 29.86 μmol/L 和 192.21 ± 46.71 μmol/L 比 128.95 ± 3.06 μmol/L，P < 0.002 和 P < 0.001）、MTs 水平（8.18 ± 0.35 μg/mL 和 9.20 ± 0.58 μg/mL 比 6.12 ± 0.25 μg/mL，P < 0.05 和 P < 0.05）和 p53Abs 水平（20.19 ± 3.20 U/mL 和 34.66 ± 1.34 U/mL 比 9.42 ± 1.64 U/mL，P < 0.001 和 P < 0.001）均显著升高。与无异型增生组相比，UC 伴异型增生患者的 AOPPs 水平（P < 0.05）和 p53Abs 水平（P < 0.001）均显著升高，而两组 MTs 水平无显著差异（P > 0.096）。相反，两组患者的 GSH 水平（1.87 ± 0.02 μmol/mL 和 1.37 ± 0.09 μmol/mL 比 2.49 ± 0.10 μmol/mL，P < 0.05 和 P < 0.05）均显著降低，且 III 组的 GSH 水平显著低于 II 组（P < 0.05）。AOPPs 与 MTs（r = 0.678，P < 0.001）和 p53Abs（r = 0.547，P < 0.001）呈正相关，p53Abs 与 MTs 呈正相关（r = 0.739，P < 0.001）。AOPPs 与 GSH（r = -0.385，P < 0.001）呈负相关，GSH 与 MTs（r = -0.662，P < 0.001）和 p53Abs（r = -0.923，P < 0.001）呈负相关。
氧化应激和氧化细胞损伤在慢性 UC 的发病机制及其相关致癌过程中发挥重要作用。p53Abs 水平有助于早期发现这些情况下的异型增生。
