Department of Structural Biology, St, Jude Children's Research Hospital, Memphis, TN 38105, USA.
Cell Commun Signal. 2010 May 28;8:8. doi: 10.1186/1478-811X-8-8.
PDZ domains are abundant protein interaction modules that often recognize short amino acid motifs at the C-termini of target proteins. They regulate multiple biological processes such as transport, ion channel signaling, and other signal transduction systems. This review discusses the structural characterization of PDZ domains and the use of recently emerging technologies such as proteomic arrays and peptide libraries to study the binding properties of PDZ-mediated interactions. Regulatory mechanisms responsible for PDZ-mediated interactions, such as phosphorylation in the PDZ ligands or PDZ domains, are also discussed. A better understanding of PDZ protein-protein interaction networks and regulatory mechanisms will improve our knowledge of many cellular and biological processes.
PDZ 结构域是丰富的蛋白质相互作用模块，通常识别靶蛋白 C 末端的短氨基酸基序。它们调节多种生物过程，如运输、离子通道信号转导和其他信号转导系统。本综述讨论了 PDZ 结构域的结构特征，以及使用最近出现的技术，如蛋白质组阵列和肽文库，来研究 PDZ 介导的相互作用的结合特性。还讨论了负责 PDZ 介导的相互作用的调节机制，如 PDZ 配体或 PDZ 结构域中的磷酸化。更好地理解 PDZ 蛋白质-蛋白质相互作用网络和调节机制将提高我们对许多细胞和生物学过程的认识。
