Mouse Imaging Centre, Hospital for Sick Children, Toronto, Ontario, Canada.
Magn Reson Med. 2010 Jun;63(6):1703-7. doi: 10.1002/mrm.22352.
MR microscopy is being explored as a useful imaging tool to phenotype mouse embryos due to its volume coverage with three-dimensional isotropic resolution. However, the main limitation for mouse embryo MR microscopy is the signal-to-noise ratio. Large numbers of embryos are needed for phenotypic screening, making high throughput essential. Two high-throughput imaging approaches, multi-embryo shared-coil (shared) and multi-embryo individual-coil (individual), have been developed for phenotyping mouse embryos. This study quantitatively compares the signal-to-noise ratio at equivalent times between these two established methods by compensating for differences that result from field strength. While the individual method provides 3.3 times as much signal-to-noise ratio as the shared method at equivalent conditions, it is more difficult and expensive to implement. Furthermore, the number of embryos that can be imaged concurrently is limited by the number of receiver channels. The objective of this study is to provide measured comparative data to guide choices for high-throughput mouse embryo MR microscopy and other similar applications.
磁共振显微镜（MR microscopy）因其具有三维各向同性分辨率的容积覆盖范围，正被探索作为一种有用的成像工具来对小鼠胚胎进行表型分析。然而，小鼠胚胎磁共振显微镜的主要限制是信噪比。进行表型筛选需要大量的胚胎，因此高通量是必不可少的。已经开发了两种高通量成像方法，多胚胎共享线圈（共享）和多胚胎独立线圈（独立），用于对小鼠胚胎进行表型分析。本研究通过补偿由于场强差异而导致的差异，定量比较了这两种已建立方法在等效时间下的信噪比。虽然在等效条件下，独立方法提供的信噪比是共享方法的 3.3 倍，但它的实施更困难且昂贵。此外，接收器通道的数量限制了可以同时成像的胚胎数量。本研究的目的是提供经过测量的比较数据，以指导高通量小鼠胚胎磁共振显微镜和其他类似应用的选择。
