Johns Hopkins Medical Institutions, Baltimore, MD 21231, USA.
Med Princ Pract. 2010;19(4):247-54. doi: 10.1159/000312709. Epub 2010 May 26.
In this postgenomic age, cancer will be understood in intricate detail beyond the genomic level. New technologies are emerging that allow global proteomic level characterization, profiling and understanding. One of the most exciting emerging technologies of cancer proteomics is protein microarray. In this review, the different types of protein microarrays are discussed, including the methods, challenges and techniques of each type. Subsequently, the application of these specific methods to cancer diagnosis, prognosis and therapy will be overviewed, providing a general review of current methods, and proposing how protein arrays will help shape the future of oncoproteomics.
在这个后基因组时代，癌症将在基因组水平之外被精细地理解。新的技术正在涌现，这些技术允许进行全局蛋白质组水平的特征描述、分析和理解。癌症蛋白质组学中最令人兴奋的新兴技术之一是蛋白质微阵列。在这篇综述中，讨论了不同类型的蛋白质微阵列，包括每种类型的方法、挑战和技术。随后，将综述这些特定方法在癌症诊断、预后和治疗中的应用，提供对当前方法的总体回顾，并提出蛋白质阵列将如何帮助塑造肿瘤蛋白质组学的未来。
