Wojtkowski Maciej
Institute of Physics, Nicolaus Copernicus University, ul. Grudziadzka 5, 87-100, Torun, Poland.
Appl Opt. 2010 Jun 1;49(16):D30-61. doi: 10.1364/AO.49.000D30.
In the past decade we have observed a rapid development of ultrahigh-speed optical coherence tomography (OCT) instruments, which currently enable performing cross-sectional in vivo imaging of biological samples with speeds of more than 100,000 A-scans/s. This progress in OCT technology has been achieved by the development of Fourier-domain detection techniques. Introduction of high-speed imaging capabilities lifts the primary limitation of early OCT technology by giving access to in vivo three-dimensional volumetric reconstructions on large scales within reasonable time constraints. As result, novel tools can be created that add new perspective for existing OCT applications and open new fields of research in biomedical imaging. Especially promising is the capability of performing functional imaging, which shows a potential to enable the differentiation of tissue pathologies via metabolic properties or functional responses. In this contribution the fundamental limitations and advantages of time-domain and Fourier-domain interferometric detection methods are discussed. Additionally the progress of high-speed OCT instruments and their impact on imaging applications is reviewed. Finally new perspectives on functional imaging with the use of state-of-the-art high-speed OCT technology are demonstrated.
在过去十年中，我们观察到超高速光学相干断层扫描（OCT）仪器迅速发展，目前这些仪器能够以超过100,000次A扫描/秒的速度对生物样本进行体内横截面成像。OCT技术的这一进展是通过傅里叶域检测技术的发展实现的。高速成像功能的引入克服了早期OCT技术的主要限制，能够在合理的时间限制内大规模地进行体内三维体积重建。因此，可以创建新的工具，为现有的OCT应用带来新的视角，并开辟生物医学成像的新研究领域。特别有前景的是进行功能成像的能力，它显示出通过代谢特性或功能反应来区分组织病理学的潜力。在本论文中，将讨论时域和傅里叶域干涉检测方法的基本限制和优点。此外，还将回顾高速OCT仪器的进展及其对成像应用的影响。最后，展示了使用最先进的高速OCT技术进行功能成像的新视角。
