Hu Kai-Zhi, Liu Jie, Xiao Bo, Liu Yan-Qin, Qin Jing, Han Ru-Gang
The Research Institute of Medicine Plantation of Chongqing, Chongqing 408435, China.
Zhong Yao Cai. 2010 Jan;33(1):7-10.
Exploring seed storage method to offer a technological support for the seedling development of Bletilla striata.
Bletilla striata seeds were stored by means of sand storage, nature storage and low temperature storage for 120, 150 and 180 d respectively. The germination rates were measured with methods of seed germination on the Petri dish and difference of rates was compared by means of the SSR method.
The embryo rate of Bletilla striata seeds was (84.9 +/- 4.7)%; the germination rate of fresh seeds was (92.1 +/- 2.1)%; the germination rates of seeds treated by sand storage, nature storage and low temperature storage for 120, 150 and 180 d were (89.3 +/- 2.7)%, (77.4 +/- 3.3)%, (68.9 +/- 2.7)% respectively and they showed significant difference or extreme significant difference one another. When seeds stored 120, 150 and 180 d, germination rates of seeds treated with three methods were (81.7 +/- 8.0)%, (78.5 +/- 7.9)%, (75.7 +/- 8.5)% respectively, and in these three storage times, germination rates of seeds stored 120 d and 180 d showed significant difference. In addition, there was no significant difference between germination rates of fresh seeds and sand storage seeds stored 120 d.
The effect of germination of seeds stored with sand was good, and germination rate was high steal after 180 d.
探索白及种子贮藏方法，为白及种苗培育提供技术支持。
采用沙藏、自然贮藏和低温贮藏分别对白及种子进行120、150和180 d的贮藏处理。通过培养皿种子发芽法测定发芽率，采用SSR法比较发芽率差异。
白及种子的胚率为(84.9±4.7)%；新鲜种子的发芽率为(92.1±2.1)%；经沙藏、自然贮藏和低温贮藏120、150和180 d的种子发芽率分别为(89.3±2.7)%、(77.4±3.3)%、(68.9±2.7)%，相互之间存在显著差异或极显著差异。种子贮藏120、150和180 d时，3种方法处理的种子发芽率分别为(81.7±8.0)%、(78.5±7.9)%、(75.7±8.5)%，在这3个贮藏时间中，贮藏120 d和180 d的种子发芽率存在显著差异。此外，新鲜种子与贮藏120 d的沙藏种子发芽率之间无显著差异。
沙藏种子的发芽效果良好，贮藏180 d后发芽率仍较高。
