Li Wei-Ping, Tian Sha-Sha, Lu Guang-Yao, Lv Di, Wang Yang-Ni, Jiang Fu-Sheng, Ding Zhi-Shan
(Zhejiang Chinese Medical University, Hangzhou 310053, China.
Zhongguo Zhong Yao Za Zhi. 2012 Nov;37(22):3386-90.
To establish a new manufacturing method for Bletilla striata synthetic seeds, and provided a new way for rapid propagation of B. striata, the correlated influential factors were studied.
The synthetic seeds were manufactured by taking seeds of B. striata as materials which were beforehand germinated in 1/2 MS medium for 10 days, and the influential factors such as artificial endosperm components, episperm substances, storage conditions and germination groundmass impact on the germination rate and seedling rate of the synthetic seeds were evaluated.
Compound 4.0% sodium alginate + 0.2 mol x L(-1) CaCl2 + 0.4 mg x L(-1) penicillin + 0.3% carbendazim powder + 0.2% sodium benzoate served as the best episperm substances while MS + 1.0 mg x L(-1) NAA + 2.0 mg x L(-1) KT as the best endosperm components, in which, high germination rate and seedling rate were obtained. The synthetic seeds storing in the 4 degrees C for a long time was able to have still high vitality.
The B. striata synthetic seeds manufacturing system was established successfully, while efforts should be taken to improve the sowing technique of the synthetic seeds in non-sterile conditions.
建立一种白及人工种子的新制作方法，为白及的快速繁殖提供新途径，并对相关影响因素进行研究。
以白及种子为材料，先在1/2 MS培养基中萌发10天，制作人工种子，评估人工胚乳成分、种皮物质、贮藏条件和萌发基质等因素对人工种子萌发率和成苗率的影响。
复合4.0%海藻酸钠+0.2 mol·L(-1)氯化钙+0.4 mg·L(-1)青霉素+0.3%多菌灵粉剂+0.2%苯甲酸钠为最佳种皮物质，MS+1.0 mg·L(-1)萘乙酸+2.0 mg·L(-1)激动素为最佳胚乳成分，在此条件下，人工种子具有较高的萌发率和成苗率。4℃长期贮藏的人工种子仍具有较高活力。
成功建立了白及人工种子制作体系，但应致力于改进人工种子在非无菌条件下的播种技术。
