Whelan J, Broughton K S, Kinsella J E
Lipids Research Laboratory, Institute of Food Science, Cornell University, Ithaca, New York 14853.
Lipids. 1991 Feb;26(2):119-26. doi: 10.1007/BF02544005.
The comparative effects of dietary alpha-linolenic acid and fish oil on eicosanoid metabolism was studied in vivo. Resident murine peritoneal cells were stimulated in vivo with opsonized zymosan in animals maintained on diets containing increasing amounts of alpha-linolenic acid or fish oil concentrate with projected n-3/n-6 ratios of 0.2, 0.4 and 1.0. While fish oil feeding resulted in significant changes in eicosapentaenoate tissue levels, alpha-linolenic acid was preferentially metabolized to docosahexaenoate. High performance liquid chromatographic analysis revealed the formation of leukotriene E5 (LTE5) in all the fish oil groups (19.8 +/- 3.5 ng/mouse to 83.3 +/- 13 ng/mouse), but only in the highest linolenic acid group (6.0 +/- 3.2 ng/mouse). Concomitantly, the 4-series sulfidopeptide leukotrienes and PGI2 were significantly reduced in the two highest fish oil containing dietary groups. Similar reductions were observed in the highest linolenic acid group, but the changes were not statistically different from the control values. In summary, this paper reports the de novo synthesis of 5-series sulfidopeptide leukotrienes in animals consuming alpha-linolenic acid. It also reveals that dietary fish oil is 2.5 to 5 times more effective than alpha-linolenic acid in modulating eicosanoid metabolism and altering tissue phospholipid fatty acid composition.
在体内研究了膳食α-亚麻酸和鱼油对类二十烷酸代谢的比较作用。在食用含α-亚麻酸或鱼油浓缩物（预计n-3/n-6比例为0.2、0.4和1.0）量不断增加的饮食的动物中，用调理酵母聚糖在体内刺激常驻小鼠腹膜细胞。虽然喂食鱼油导致二十碳五烯酸组织水平发生显著变化，但α-亚麻酸优先代谢为二十二碳六烯酸。高效液相色谱分析显示，所有鱼油组均形成白三烯E5（LTE5）（19.8±3.5 ng/小鼠至83.3±13 ng/小鼠），但仅在最高亚麻酸组中形成（6.0±3.2 ng/小鼠）。同时，在两个最高鱼油含量的饮食组中，4-系列硫化肽白三烯和PGI2显著降低。在最高亚麻酸组中也观察到类似的降低，但变化与对照值无统计学差异。总之，本文报道了食用α-亚麻酸的动物中5-系列硫化肽白三烯的从头合成。它还表明，膳食鱼油在调节类二十烷酸代谢和改变组织磷脂脂肪酸组成方面比α-亚麻酸有效2.5至5倍。
