Wnt5a 促进人牙髓细胞的分化。
Wnt5a promotes differentiation of human dental papilla cells.
机构信息
West China Hospital of Stomatology, Sichuan University, Chengdu, China.
出版信息
Int Endod J. 2010 May;43(5):404-12. doi: 10.1111/j.1365-2591.2010.01693.x.
AIM
To investigate the role of Wnt5a in the process of differentiation of human dental papilla cells (HDPCs).
METHODOLOGY
Recombinant adenovirus encoding full-length Wnt5a cDNA was constructed to investigate the biological role of Wnt5a on the differentiation of HDPCs. The effect of Wnt5a on HDPCs differentiation was determined by ALP activity assay, ALP staining and mineral induction assay. Mineralization-related gene expressions were assessed by RT-PCR.
RESULTS
Immunostaining revealed Wnt5a expression in the odontoblast layer and dental papilla tissue. Over-expression of Wnt5a by transfecting HDPCs with an Wnt5a-carrying construct increased ALPase activity and the formation of mineralized nodules of HDPCs. RT-PCR analysis showed that the expressions of mineralization-related genes, such as bone sialoprotein, collagen type I, osteonectin, osteopontin (OCN), dentine matrix protein-1 were up-regulated by Wnt5a.
CONCLUSIONS
Wnt5a promoted differentiation of HDPCs.
目的
研究 Wnt5a 在人牙髓细胞（HDPCs）分化过程中的作用。
方法
构建了编码全长 Wnt5a cDNA 的重组腺病毒，以研究 Wnt5a 对 HDPCs 分化的生物学作用。通过碱性磷酸酶（ALP）活性测定、ALP 染色和矿化诱导试验来确定 Wnt5a 对 HDPCs 分化的影响。通过 RT-PCR 评估矿化相关基因的表达。
结果
免疫组化显示 Wnt5a 在成牙本质细胞层和牙髓组织中表达。用携带 Wnt5a 的构建体转染 HDPCs 可过表达 Wnt5a，从而增加 HDPCs 的 ALP 酶活性和矿化结节的形成。RT-PCR 分析表明，Wnt5a 上调了骨涎蛋白、Ⅰ型胶原、骨连接蛋白、骨桥蛋白（OCN）、牙本质基质蛋白-1 等矿化相关基因的表达。
结论
Wnt5a 促进了 HDPCs 的分化。
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