Maerker G, Jones K C
U.S. Department of Agriculture, Eastern Regional Research Center, Philadelphia, Pennsylvania 19118.
Lipids. 1991 Feb;26(2):139-44. doi: 10.1007/BF02544008.
Cholesterol in aqueous suspensions of multilamellar vesicles (MLV) was exposed to gamma-irradiation (0.5-10 kGy) at 0-4 degrees C. Cholesterol oxidation products resulting from the irradiation were isolated by dry column extraction followed by preparative thin-layer chromatography (TLC) and were quantitated by on-column gas chromatography (GC). The ratio of 7-ketocholesterol/cholesterol 5,6-epoxides generated by irradiation was less than one, much lower than the ratio of ten commonly produced by autoxidation. Irradiation also produced relatively higher amounts of 7-hydroxycholesterol than did autoxidation. These unique product ratios may be suitable indicators of past exposure to irradiation.
多层囊泡（MLV）水悬浮液中的胆固醇在0 - 4摄氏度下接受γ辐射（0.5 - 10千戈瑞）。辐照产生的胆固醇氧化产物通过干柱萃取，然后进行制备型薄层色谱（TLC）分离，并通过柱上气相色谱（GC）进行定量。辐照产生的7 - 酮胆固醇/胆固醇5,6 - 环氧化物的比例小于1，远低于自氧化通常产生的10的比例。与自氧化相比，辐照还产生了相对较高量的7 - 羟基胆固醇。这些独特的产物比例可能是过去暴露于辐照的合适指标。
