pr-M 裂解对细胞外登革病毒粒子异质性的影响。
Influence of pr-M cleavage on the heterogeneity of extracellular dengue virus particles.
机构信息
Department of Microbiology, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand.
出版信息
J Virol. 2010 Aug;84(16):8353-8. doi: 10.1128/JVI.00696-10. Epub 2010 Jun 2.
Abstract
During dengue virus replication, an incomplete cleavage of the envelope glycoprotein prM, generates a mixture of mature (prM-less) and prM-containing, immature extracellular particles. In this study, sequential immunoprecipitation and cryoelectron microscopy revealed a third type of extracellular particles, the partially mature particles, as the major prM-containing particles in a dengue serotype 2 virus. Changes in the proportion of viral particles in the pr-M junction mutants exhibiting altered levels of prM cleavage suggest that the partially mature particles may represent an intermediate subpopulation in the virus maturation pathway. These findings are consistent with a model suggesting the progressive mode of prM cleavage.
摘要
在登革热病毒复制过程中，包膜糖蛋白 prM 的不完全裂解会产生成熟（无 prM）和含有 prM 的不成熟细胞外颗粒的混合物。在这项研究中，连续免疫沉淀和冷冻电子显微镜揭示了第三种细胞外颗粒，即部分成熟颗粒，是登革热病毒 2 型的主要含 prM 颗粒。在表现出不同 prM 切割水平的 pr-M 连接突变体中，病毒颗粒比例的变化表明，部分成熟颗粒可能代表病毒成熟途径中的中间亚群。这些发现与渐进式 prM 切割模型一致。
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