多基因座可变数目串联重复分析在流行地区对医院获得性耐甲氧西林金黄色葡萄球菌的多克隆菌株进行分型的评价。
Evaluation of multiple-locus variable-number tandem-repeat analysis for typing a polyclonal hospital-acquired methicillin-resistant Staphylococcus aureus population in an area where such infections are endemic.
机构信息
Laboratorio de Biología Molecular, Unidad de Investigación, Hospital Universitario Nuestra Señora de Candelaria, Santa Cruz de Tenerife, Spain.
出版信息
J Clin Microbiol. 2010 Aug;48(8):2991-4. doi: 10.1128/JCM.02220-09. Epub 2010 Jun 2.
Abstract
Multiple-locus variable-number tandem-repeat analysis (MLVA) was performed with 292 methicillin-resistant Staphylococcus aureus (MRSA) isolates previously characterized by pulsed-field gel electrophoresis, multilocus sequence typing, and staphylococcal cassette chromosome mec typing. Quantitative correspondence analyses showed the best correlation between data when an >or=80% cutoff was applied to MLVA. We confirmed the validity of MLVA for identification of related strains in a polyclonal MRSA population.
摘要
采用多位点可变数目串联重复分析（MLVA）对 292 株先前经脉冲场凝胶电泳、多位点序列分型和葡萄球菌盒式染色体 mec 分型鉴定的耐甲氧西林金黄色葡萄球菌（MRSA）进行分析。定量对应分析显示，当 MLVA 应用 >或=80%的截断值时，数据相关性最佳。我们在多克隆 MRSA 群体中证实了 MLVA 用于鉴定相关菌株的有效性。
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