Graduate School of East-West Medical Science, Kyung Hee University, Yongin-si, Republic of Korea.
J Med Food. 2010 Jun;13(3):673-80. doi: 10.1089/jmf.2009.1239.
Ultraviolet (UV) light is a physical carcinogen, and UV irradiation from sunlight has negative effects on human skin. UVB-induced inflammation is linked to excessive induction of various inflammatory cytokines/chemokines in many types of cells, including keratinocytes. The purpose of this study was to investigate the anti-inflammatory effect of water extract of Zingiber officinale, gingerol, and shogaol on UVB-induced skin damage in the human keratinocyte cell line HaCaT and C57BL/6 mice. To test for an effective compound to protect against inflammation in UV-damaged skin, we prepared a water extract of ginger rhizomes and examined the effects of Z. officinale, gingerol, and shogaol on cell viability and cytokine/chemokine production in UV-irradiated HaCaT cells. We also investigated the in vivo relevance of these findings in C57BL/6 mice using hematoxylin and eosin staining and cytokine measurements. A water extract of Z. officinale, gingerol, and shogaol inhibited production of cytokines in UVB-irradiated HaCaT cells effectively. Treatment with Z. officinale attenuated UVB-induced hyperplasia, infiltration of leukocytes, and dilation of blood vessels in the dermis of mice. Z. officinale, gingerol, and shogaol show potential as anti-inflammatory agents to protect skin against UVB irradiation damage.
紫外线（UV）光是一种物理致癌物质，阳光中的 UV 辐射对人类皮肤有负面影响。UVB 诱导的炎症与多种细胞（包括角质形成细胞）中各种炎症细胞因子/趋化因子的过度诱导有关。本研究旨在探讨生姜水提取物、姜酚和姜酮对 UVB 诱导的人角质形成细胞系 HaCaT 和 C57BL/6 小鼠皮肤损伤的抗炎作用。为了寻找一种有效的化合物来预防 UV 损伤皮肤的炎症，我们制备了生姜根茎的水提取物，并研究了 Z. officinale、姜酚和姜酮对 UV 照射的 HaCaT 细胞活力和细胞因子/趋化因子产生的影响。我们还使用苏木精和伊红染色和细胞因子测量法研究了这些发现对 C57BL/6 小鼠的体内相关性。生姜水提取物、姜酚和姜酮可有效抑制 UVB 照射的 HaCaT 细胞中细胞因子的产生。Z. officinale 处理可减轻 UVB 诱导的小鼠真皮中细胞增生、白细胞浸润和血管扩张。Z. officinale、姜酚和姜酮具有作为抗炎剂的潜力，可保护皮肤免受 UVB 照射损伤。
