Departamento de Microbiología del Suelo y Sistemas Simbióticos, Estación Experimental del Zaidín, CSIC, Profesor Albareda 1, 18008 Granada, Spain.
Mycologia. 2010 May-Jun;102(3):624-32. doi: 10.3852/09-145.
A new fungal species in the arbuscular mycorrhiza-forming Glomeromycetes, Entrophospora nevadensis, was isolated from soil near the roots of several endemic and endangered plant species (e.g. Plantago nivalis and Alchemilla fontqueri) growing in Sierra Nevada National Park (Granada, Andalucia, Spain). The fungus was propagated in trap cultures on Plantago nivalis and Sorbus hybrida and in pure cultures on Trifolium pratense and Sorghum vulgare. Spores are yellow brown to brown, 90-115 .m diam and form singly in soil, in the neck of adherent sporiferous saccules that form either terminally or intercalary on mycelial hyphae. Spores have two three-layered walls and conspicuous, 6-12 microm long, spiny, thorn-like projections on the outer wall consisting of hyaline to subhyaline, evanescent tips and yellow brown to brown, persistent bases. In aging spores these projections are usually shorter (1-2.8 microm) and dome-shaped or rounded, sometimes with a central pit on top where the evanescent tip has sloughed off. Molecular analysis with partial sequences of the 18S ribosomal gene places the fungus within the Diversisporales. The new fungus was found in soil near plants with different living strategies but growing in high altitude soils with acidic pH, high soil moisture and organic carbon content, and close to streams.
一种新的丛枝菌根真菌 Entrophospora nevadensis，从生长在西班牙内华达山脉国家公园（格拉纳达，安达卢西亚）的几种特有和濒危植物（如高山火绒草和Fontqueri 委陵菜）根际土壤中分离出来。该真菌在高山火绒草和杂种鹅耳枥的诱捕培养物以及三叶草和高粱的纯培养物中繁殖。孢子呈黄棕色至棕色，直径 90-115 µm，单独存在于土壤中，存在于附着的孢子囊的颈部，孢子囊在菌丝上末端或中间形成。孢子有两层三层壁和明显的、6-12 µm 长的、带刺的、刺状突起在外壁上，由透明到亚透明、消失的尖端和黄棕色到棕色、持久的基部组成。在老化的孢子中，这些突起通常较短（1-2.8 µm），呈圆顶状或圆形，有时在顶部有一个中央坑，消失的尖端已经脱落。用 18S 核糖体基因的部分序列进行的分子分析将真菌置于 Diversisporales 内。新真菌在不同生活策略的植物附近的土壤中发现，但生长在高海拔土壤中，这些土壤具有酸性 pH 值、高土壤湿度和有机碳含量，并且靠近溪流。
