Department of Plant Pathology, Shandong Agricultural University, Taiwan, 271018, China.
Mycologia. 2010 May-Jun;102(3):708-17. doi: 10.3852/09-208.
Two new species of Stemphylium (anamorphic Pleospora) are described on the basis of morphological characters and molecular phylogenetic analyses. Stemphylium phaseolina and S. variabilis were isolated respectively from diseased leaves of Phaseolus vulgaris L. in Hebei Province, China, and from diseased leaves of Allium sativum L. in Angres, France. The two species exhibit characteristic Stemphylium morphology but are distinct from similar species based on the morphology and development of conidia. The internal transcribed spacer (ITS) nuclear rDNA region and glyceraldehyde-3-phosphate dehydrogenase (gpd) genes were sequenced. The results of maximum parsimony (MP) and maximum likelihood (ML) phylogenetic analyses of the combined DNA sequences of these two gene regions supported S. phaseolina and S. variabilis as two distinct phylogenetic species. The taxonomic descriptions of the new species and their comparison with related species are presented, together with the phylogenetic analysis based on combined DNA sequences of ITS and gpd gene regions.
两种新的节丛孢属（拟茎点霉属）真菌基于形态特征和分子系统发育分析进行了描述。茎点霉属菜豆叶斑病菌和节丛孢属洋葱叶斑病菌分别从中国河北省的菜豆叶片病害和法国昂格雷的洋葱叶片病害中分离得到。这两个种表现出典型的节丛孢属形态特征，但基于分生孢子的形态和发育与相似种明显不同。对ITS 核 rDNA 区和甘油醛-3-磷酸脱氢酶（gpd）基因进行了测序。ITS 和 gpd 基因区域联合 DNA 序列的最大简约法（MP）和最大似然法（ML）系统发育分析结果支持茎点霉属菜豆叶斑病菌和节丛孢属洋葱叶斑病菌是两个不同的系统发育种。本文对新种进行了分类描述，并与相关种进行了比较，同时还基于 ITS 和 gpd 基因区域联合 DNA 序列进行了系统发育分析。
