Aktar-Uz-Zaman Md, Haque Md Ashraful, Sarker Ashutosh, Rohman Md Motiar, Sarkar Debashish, Muzahid-E-Rahman Md, Haque Ruan Binte, Akhter Md Shamim, Agrawal Shiv Kumar
Pulses Breeding, Pulses Research Centre, Bangladesh Agricultural Research Institute (BARI), Ishurdi, Pabna, 6620, Bangladesh.
Department of Genetics and Plant Breeding, Bangladesh Agricultural University (BAU), Mymensingh, Bangladesh.
Sci Rep. 2025 Sep 2;15(1):32318. doi: 10.1038/s41598-025-15587-4.
During the cropping seasons of 2019-20, 2020-21, and 2021-22, respectively, lentil genotypes were screened against Stemphylium blight using the Alpha-Lattice Design with three replications at Pulses Research Centre, BARI, Ishurdi, Pabna-6620 under natural and artificial epiphytotic conditions. In case of artificial inoculum condition, the artificial cultured inoculums were sprayed at 1.3 × 10 spores/mL concentration of spores for spore inoculation in the experimental plots. The disease severity data was recorded following 1-9 disease rating scale and Area under Disease Progress Curve (AUDPC) was also estimated. Considering the disease severity and Area under Disease Progress Curve (AUDPC) under natural epiphytotic condition none of the genotype was identified as Resistant, and Very Susceptible but seven genotypes namely BARI Masur-9, BARI Masur-8, PRECOZ, BLX-12004-5, RL-12-181, BLX 10001-1, and BLX 09015 were found as Moderately Resistance-Moderately Susceptible genotypes against Stemphylium blight disease with disease rating scale 4, 16 to 30% disease infection rate, and average estimated AUDPC 81-120 among the 60 lentil genotypes. But in case of artificial inoculation during cropping season 2021-22, only three genotypes BARI Masur-9, PRECOZ, and BLX 09015 were identified as Moderately Susceptible. Disease severity was recorded higher in artificial inoculation compared to natural epiphytotic condition. Considering the yield performance under natural and artificial inoculation the genotypes BARI Masur-9, PRECOZ, BARI Masur-8, LRIL-21-112-1-1-1-1-6, BLX-12004-5, BLX 10001-1, BLX 09015, and RL-12-181 were identified Moderately-Resistant to Moderately-Susceptible genotypes with better yield performance under disease infestation.
在2019 - 20、2020 - 21和2021 - 22年的种植季节，分别在帕布纳伊舒尔迪巴里脉冲研究中心，采用α - 格子设计，在自然和人工流行条件下对小扁豆基因型进行了三次重复的针对匍柄霉叶枯病的筛选。在人工接种条件下，将人工培养的接种物以1.3×10个孢子/毫升的孢子浓度喷洒在试验田中进行孢子接种。按照1 - 9级病害评级标准记录病害严重程度数据，并估算病害进展曲线下面积（AUDPC）。考虑到自然流行条件下的病害严重程度和病害进展曲线下面积（AUDPC），没有基因型被鉴定为抗病和极感病，但在60个小扁豆基因型中，有7个基因型，即BARI Masur - 9、BARI Masur - 8、PRECOZ、BLX - 12004 - 5、RL - 12 - 181、BLX 10001 - 1和BLX 09015，被发现是对匍柄霉叶枯病具有中抗 - 中感的基因型，病害评级为4级，病害感染率为16%至30%，平均估算AUDPC为81 - 120。但在2021 - 22年种植季节人工接种的情况下，只有3个基因型BARI Masur - 9、PRECOZ和BLX 09015被鉴定为中感。与自然流行条件相比，人工接种时记录的病害严重程度更高。考虑到自然和人工接种条件下的产量表现，基因型BARI Masur - 9、PRECOZ、BARI Masur - 8、LRIL - 21 - 112 - 1 - 1 - 1 - 1 - 6、BLX - 12004 - 5、BLX 10001 - 1、BLX 09015和RL - 12 - 181被鉴定为中抗 - 中感基因型，在病害侵染下具有较好的产量表现。
