Department of Chemistry & Maurice Morton Institute of Polymer Science, The University of Akron, Akron, Ohio 44325, USA.
J Phys Chem B. 2010 Jul 1;114(25):8574-80. doi: 10.1021/jp1029536.
A series of squaraine dyes are found to be in H- or J-aggregates and are almost nonfluorescent in aqueous solution. Upon addition of bovine serum albumin (BSA), however, the fluorescence intensity (at lambda(em) approximately 690 nm) increases by a factor of up to 200. Transformation of the dye molecules from the aggregates to the monomeric species appears to be responsible for the large fluorescence turn-on. While both H- and J-aggregates contribute to the observed fluorescence turn-on, the former appears to play a more important role. Electrophoresis imaging shows that these probes are good BSA indicators.
一系列方酸染料被发现处于 H 或 J 聚集态，在水溶液中几乎没有荧光。然而，当加入牛血清白蛋白（BSA）时，荧光强度（在 lambda(em)约 690nm 处）增加了 200 倍。染料分子从聚集态到单体态的转变似乎是导致荧光开启的原因。虽然 H 聚集态和 J 聚集态都对观察到的荧光开启有贡献，但前者似乎起着更重要的作用。电泳成像显示这些探针是良好的 BSA 指示剂。
