School of Life Science, Beijing Institute of Technology, Beijing 100081, PR China.
China General Microbiological Culture Collection Center, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
Int J Syst Evol Microbiol. 2011 May;61(Pt 5):1078-1083. doi: 10.1099/ijs.0.023671-0. Epub 2010 Jun 4.
A Gram-stain-positive, rod-shaped bacterium, designated strain NH3(T), was isolated from a sediment sample from the South China Sea and was subjected to a polyphasic taxonomic study. The isolate grew optimally at 37 °C and pH 9. Strain NH3(T) had cell-wall peptidoglycan based on meso-diaminopimelic acid and MK-7 as the predominant menaquinone. The cellular fatty acid profile included significant amounts of iso-C(15 : 0) and iso-C(14 : 0). The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The DNA G+C content of strain NH3(T) was 40.3 mol%. Phylogenetic analysis of the 16S rRNA gene sequence revealed that strain NH3(T) was a member of rRNA group 6 of the genus Bacillus, which includes alkalitolerant, alkaliphilic and halotolerant species. The closest phylogenetic relatives were Bacillus akibai 1139(T) (96.82 % 16S rRNA gene sequence similarity), B. pseudofirmus DSM 8715(T) (96.76 %), B. okhensis Kh10-101(T) (96.76 %) and B. alkalidiazotrophicus MS 6(T) (96.47 %). Strain NH3(T) could be distinguished from these phylogenetically close neighbours based on a number of phenotypic properties. On the basis of phenotypic and chemotaxonomic characteristics and phylogenetic data, we conclude that strain NH3(T) ( = CGMCC 1.10116(T) = JCM 16507(T)) merits classification as the type strain of a novel species, for which the name Bacillus nanhaiisediminis sp. nov. is proposed.
一株革兰氏阳性、杆状细菌，命名为 NH3(T) 菌株，从南海沉积物样本中分离得到，并进行了多相分类学研究。该分离物在 37°C 和 pH 9 下生长最佳。NH3(T) 菌株的细胞壁肽聚糖基于内消旋二氨基庚二酸和 MK-7 作为主要的menaquinone。细胞脂肪酸谱包括大量的异-C(15:0)和异-C(14:0)。主要的极性脂包括磷脂酰乙醇胺、磷脂酰甘油和双磷脂酰甘油。NH3(T) 菌株的 DNA G+C 含量为 40.3 mol%。16S rRNA 基因序列的系统发育分析表明，NH3(T) 菌株是芽孢杆菌属 rRNA 组 6 的成员，其中包括耐碱、嗜碱和耐盐的种。最接近的系统发育亲缘关系是 Bacillus akibai 1139(T)(96.82%16S rRNA 基因序列相似性)、B. pseudofirmus DSM 8715(T)(96.76%)、B. okhensis Kh10-101(T)(96.76%)和 B. alkalidiazotrophicus MS 6(T)(96.47%)。NH3(T) 菌株可以根据许多表型特性与这些亲缘关系密切的近缘种区分开来。基于表型和化学分类特征以及系统发育数据，我们得出结论，NH3(T) 菌株（ = CGMCC 1.10116(T) = JCM 16507(T)）值得分类为一个新种的模式菌株，建议将其命名为南海栖盐短小芽孢杆菌（Bacillus nanhaiisediminis）sp. nov.。
