Jin Pin-Jiao, Sun Lei, Liu Yong-Hong, Wang Kang-Kang, Narsing Rao Manik Prabhu, Mohamad Osama Abdalla Abdelshafy, Fang Bao-Zhu, Li Li, Gao Lei, Li Wen-Jun, Wang Shuang
Heilongjiang Academy of Black Soil Conservation and Utilization, Postdoctoral Station of Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China.
State Key Laboratory of Desert and Oasis Ecology, Key Laboratory of Ecological Safety and Sustainable Development in Arid Lands, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi 830011, China.
Microorganisms. 2024 May 8;12(5):950. doi: 10.3390/microorganisms12050950.
The degradation of farmland in China underscores the need for developing and utilizing saline-alkali soil. Soil health relies on microbial activity, which aids in the restoration of the land's ecosystem, and hence it is important to understand microbial diversity. In the present study, two Gram-stain-positive strains HR 1-10 and J-A-003 were isolated from saline-alkali soil. Preliminary analysis suggested that these strains could be a novel species. Therefore, the taxonomic positions of these strains were evaluated using polyphasic analysis. Phylogenetic and 16S rRNA gene sequence analysis indicated that these strains should be assigned to the genus . Cell wall contained -2,6-diaminopimelic acid. The polar lipids present in both strains were diphosphatidyl-glycerol, phosphatidylglycerol, and an unidentified phospholipid. The major fatty acids (>10%) were anteiso-C, C and iso-C. Average nucleotide identity and digital DNA#x2013;DNA hybridization values were below the threshold values (95% and 70%, respectively) for species delineation. Based on the above results, the strains represent two novel species of the genus , for which the names sp. nov., and sp. nov., are proposed. The type strains are HR 1-10 (=GDMCC 1.2946 = MCCC 1K08312 = JCM 36285), and J-A-003 (=GDMCC 1.2949 = MCCC 1K08417 = JCM 36286).
中国农田的退化凸显了开发和利用盐碱地的必要性。土壤健康依赖于微生物活动，微生物活动有助于恢复土地的生态系统，因此了解微生物多样性很重要。在本研究中，从盐碱土中分离出两株革兰氏阳性菌株HR 1-10和J-A-003。初步分析表明这些菌株可能是一个新物种。因此，使用多相分析评估这些菌株的分类地位。系统发育和16S rRNA基因序列分析表明这些菌株应归属于该属。细胞壁含有-2,6-二氨基庚二酸。两株菌株中存在的极性脂质为双磷脂酰甘油、磷脂酰甘油和一种未鉴定的磷脂。主要脂肪酸（>10%）为anteiso-C、C和iso-C。平均核苷酸同一性和数字DNA-DNA杂交值低于物种界定的阈值（分别为95%和70%）。基于以上结果，这些菌株代表了该属的两个新物种，为此提出了新物种名称sp. nov.和sp. nov.。模式菌株分别为HR 1-10（=GDMCC 1.2946 = MCCC 1K08312 = JCM 36285）和J-A-003（=GDMCC 1.2949 = MCCC 1K08417 = JCM 36286）。
