应用 TaqMan 荧光探针实时 PCR 检测和定量珊瑚病原体哈维氏弧菌
Detection and quantification of the coral pathogen Vibrio coralliilyticus by real-time PCR with TaqMan fluorescent probes.
机构信息
Australian Institute of Marine Science, PMB3, Townsville 4810, Australia.
出版信息
Appl Environ Microbiol. 2010 Aug;76(15):5282-6. doi: 10.1128/AEM.00330-10. Epub 2010 Jun 4.
Abstract
A real-time quantitative PCR-based detection assay targeting the dnaJ gene (encoding heat shock protein 40) of the coral pathogen Vibrio coralliilyticus was developed. The assay is sensitive, detecting as little as 1 CFU per ml in seawater and 10(4) CFU per cm(2) of coral tissue. Moreover, inhibition by DNA and cells derived from bacteria other than V. coralliilyticus was minimal. This assay represents a novel approach to coral disease diagnosis that will advance the field of coral disease research.
摘要
本研究开发了一种基于实时定量 PCR 的检测方法，用于检测珊瑚病原体溶珊瑚弧菌的 dnaJ 基因（编码热休克蛋白 40）。该方法具有较高的灵敏度，在海水中可检测到低至 1 CFU/ml，在珊瑚组织中可检测到低至 10^4 CFU/cm^2。此外，来自除溶珊瑚弧菌以外的细菌的 DNA 和细胞对检测的抑制作用极小。该检测方法为珊瑚疾病诊断提供了一种新方法，将推动珊瑚疾病研究领域的发展。
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本文引用的文献
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