Ghassemi S, Rossier O, Sheetz M P, Wind S J, Hone J
Department of Mechanical Engineering, Columbia University, New York, NY 10027.
J Vac Sci Technol B Microelectron Nanometer Struct Process Meas Phenom. 2009 Nov 1;27(6):3088-3091. doi: 10.1116/1.3259953.
We describe a technique for the fabrication of arrays of elastomeric pillars whose top surfaces are treated with selective chemical functionalization to promote cellular adhesion in cellular force transduction experiments. The technique involves the creation of a rigid mold consisting of arrays of circular holes into which a thin layer of Au is deposited while the top surface of the mold and the sidewalls of the holes are protected by a sacrificial layer of Cr. When an elastomer is formed in the mold, the Au adheres to the tops of the molded pillars. This can then be selectively functionalized with a protein that induces cell adhesion, while the rest of the surface is treated with a repellent substance. An additional benefit is that the tops of the pillars can be fluorescently labeled for improved accuracy in force transduction measurements.
我们描述了一种制造弹性体柱阵列的技术，其顶面经过选择性化学功能化处理，以促进细胞力转导实验中的细胞粘附。该技术包括创建一个由圆孔阵列组成的刚性模具，在模具顶表面和孔的侧壁由Cr牺牲层保护的情况下，向其中沉积一层薄金。当在模具中形成弹性体时，金附着在模制柱的顶部。然后可以用诱导细胞粘附的蛋白质对其进行选择性功能化，而表面的其余部分则用排斥性物质处理。另一个好处是柱的顶部可以进行荧光标记，以提高力转导测量的准确性。
