Ghassemi S, Biais N, Maniura K, Wind S J, Sheetz M P, Hone J
Department of Mechanical Engineering, Columbia University, New York, NY 10027.
J Vac Sci Technol B Microelectron Nanometer Struct Process Meas Phenom. 2008 Nov 1;26(6):2549-2553. doi: 10.1116/1.3013424.
The mechanical properties of a cell's environment can alter behavior such as migration and spreading, and control the differentiation path of stem cells. Here we describe a technique for fabricating substrates whose rigidity can be controlled locally without altering the contact area for cell spreading. The substrates consist of elastomeric pillar arrays in which the top surface is uniform but the pillar height is changed across a sharp step. Preliminary results demonstrate the effects on cell migration and morphology at the step boundary.
细胞所处环境的力学特性能够改变诸如迁移和铺展等行为，并控制干细胞的分化路径。在此，我们描述一种制造基底的技术，该基底的刚度可在不改变细胞铺展接触面积的情况下进行局部控制。这些基底由弹性柱阵列组成，其中顶面是均匀的，但柱高在一个陡峭的台阶处发生变化。初步结果表明了在台阶边界处对细胞迁移和形态的影响。
