Division of Applied Life Science (BK21 Program), Environmental Biotechnology National Core Research Center and PMBBRC, Graduate School of Gyeongsang National University, Jinju 660-701, Korea.
Plant Cell Rep. 2010 Aug;29(8):905-15. doi: 10.1007/s00299-010-0876-y. Epub 2010 Jun 5.
The phytocystatins of plants are members of the cystatin superfamily of proteins, which are potent inhibitors of cysteine proteases. The Arabidopsis genome encodes seven phytocystatin isoforms (AtCYSs) in two distantly related AtCYS gene clusters. We selected AtCYS1 and AtCYS2 as representatives for each cluster and then generated transgenic plants expressing the GUS reporter gene under the control of each gene promoter. These plants were used to examine AtCYS expression at various stages of plant development and in response to abiotic stresses. Histochemical analysis of AtCYS1 promoter- and AtCYS2 promoter-GUS transgenic plants revealed that these genes have similar but distinct spatial and temporal expression patterns during normal development. In particular, AtCYS1 was preferentially expressed in the vascular tissue of all organs, whereas AtCYS2 was expressed in trichomes and guard cells in young leaves, caps of roots, and in connecting regions of the immature anthers and filaments and the style and stigma in flowers. In addition, each AtCYS gene has a unique expression profile during abiotic stresses. High temperature and wounding stress enhanced the expression of both AtCYS1 and AtCYS2, but the temporal and spatial patterns of induction differed. From these data, we propose that these two AtCYS genes play important, but distinct, roles in plant development and stress responses.
植物中的植物胱抑素是半胱氨酸蛋白酶抑制剂的半胱氨酸蛋白酶抑制剂超家族蛋白的成员。拟南芥基因组在两个远缘 AtCYS 基因簇中编码七个植物胱抑素同工型（AtCYSs）。我们选择 AtCYS1 和 AtCYS2 作为每个簇的代表，然后生成表达 GUS 报告基因的转基因植物，该基因受每个基因启动子的控制。这些植物用于在植物发育的各个阶段以及对非生物胁迫的反应中检查 AtCYS 表达。AtCYS1 启动子和 AtCYS2 启动子-GUS 转基因植物的组织化学分析表明，这些基因在正常发育过程中具有相似但不同的时空表达模式。特别是，AtCYS1 在所有器官的血管组织中优先表达，而 AtCYS2 在幼叶、根帽、未成熟花粉和花丝的连接区以及花中的柱头和柱头中表达。此外，每个 AtCYS 基因在非生物胁迫下都有独特的表达谱。高温和创伤胁迫增强了 AtCYS1 和 AtCYS2 的表达，但诱导的时空模式不同。根据这些数据，我们提出这两个 AtCYS 基因在植物发育和应激反应中发挥着重要但不同的作用。
