Department of Neurosurgery, Chinese PLA General Hospital, Beijing, China.
J Mol Histol. 2010 Apr;41(2-3):165-76. doi: 10.1007/s10735-010-9273-7. Epub 2010 Jun 5.
Studies indicated that many tissues could express FSH. New functions of FSH have been recognized beyond reproduction regulation. However, no report has been made about the expression and function of FSH in rat spinal cord. Double-labeled immunofluorescence stain and in situ hybridization were used to study the co-localization of FSH with its receptor and co-localization of FSH with GnRH receptor in rat spinal cord. Spinal cord ischemia injury models were built, TUNEL stain and Fas immunostaining were made to observe the anti-apoptotic effects of FSH to neurons induced by spinal cord ischemia injury. The results found that some neurons and glias of rat spinal cord showed both FSH immunoreactivity and FSH mRNA positive signals; not only FSH and its receptor but also FSH and GnRH receptor co-located in cells of both gray matter and white matter; treatment with certain concentration of FSH before ischemia-reperfusion injury, less TUNEL positive cells and Fas positive cells were found in motor neurons of ventral gray matter in FSH experiment group than that in control group. These suggested that rat spinal cord could express FSH, it is also a target organ of FSH; FSH might exert functions through its receptor by paracrine or autocrine effects; GnRH in spinal cord might regulate FSH positive neurons through GnRH receptor; FSH might inhibit ischemia induced neuron apoptosis by down-regulating Fas expression in spinal cord.
研究表明，许多组织都能表达 FSH。除了生殖调控之外，FSH 的新功能也已得到确认。然而，尚未有关于 FSH 在大鼠脊髓中的表达和功能的报道。本研究采用双标免疫荧光染色和原位杂交技术，研究 FSH 与其受体以及 FSH 与 GnRH 受体在大鼠脊髓中的共定位。构建脊髓缺血损伤模型，采用 TUNEL 染色和 Fas 免疫组化，观察 FSH 对脊髓缺血损伤诱导的神经元凋亡的保护作用。结果发现，大鼠脊髓内的一些神经元和神经胶质细胞均显示 FSH 免疫反应性和 FSH mRNA 阳性信号；FSH 及其受体不仅共定位于灰质和白质的细胞中，而且 FSH 与 GnRH 受体也共定位于细胞中；在缺血再灌注损伤前给予一定浓度的 FSH 处理，FSH 实验组腹侧灰质运动神经元中的 TUNEL 阳性细胞和 Fas 阳性细胞较对照组明显减少。这些结果表明，大鼠脊髓可以表达 FSH，它也是 FSH 的靶器官；FSH 可能通过旁分泌或自分泌作用通过其受体发挥作用；脊髓中的 GnRH 可能通过 GnRH 受体调节 FSH 阳性神经元；FSH 可能通过下调脊髓中 Fas 的表达来抑制缺血诱导的神经元凋亡。
