Department of Neurosurgery, Beijing, China.
J Mol Histol. 2013 Feb;44(1):19-26. doi: 10.1007/s10735-012-9449-4. Epub 2012 Sep 13.
The expression of follicle-stimulating hormone (FSH) and its receptor in extrapituitary and non-HPG axis tissues has been demonstrated and their non-reproductive functions in these tissues have been found. However, there have been no reports concerning the expression and function of FSH and its receptor in the cerebellum. In our study, immunofluorescence staining and in situ hybridization were used to detect the expression of FSH, double-labeled immunofluorescence staining was used to detect co-localization of FSH and its receptor and co-localization of FSH and gonadotropin-releasing hormone (GnRH) receptor in the rat cerebellar cortex. Results showed that some cells of the Purkinje cell layer, granular layer, and molecular layer of the cerebellar cortex showed both FSH immunoreactivity and FSH mRNA positive signals; not only for FSH and FSH receptor, but also for FSH and GnRH receptor co-localized in some cells throughout the Purkinje cell layer, granular layer, and molecular layer of the cerebellar cortex. These suggested that rat cerebellum could express FSH; cerebellum is a target tissue of FSH; FSH may exert certain functions through FSH receptor in a paracrine or autocrine manner; GnRH may regulate FSH positive cells through GnRH receptor in the cerebellum. Our study provides morphological evidence for further functional research on FSH and related hormones in the cerebellum.
卵泡刺激素（FSH）及其受体在垂体外和非 HPG 轴组织中的表达已经得到证实，并且在这些组织中发现了它们的非生殖功能。然而，关于 FSH 及其受体在小脑中的表达和功能还没有报道。在我们的研究中，使用免疫荧光染色和原位杂交来检测 FSH 的表达，使用双标记免疫荧光染色来检测 FSH 及其受体的共定位以及 FSH 和促性腺激素释放激素（GnRH）受体在大鼠小脑皮质中的共定位。结果表明，小脑皮质的浦肯野细胞层、颗粒层和分子层中的一些细胞表现出 FSH 免疫反应性和 FSH mRNA 阳性信号；不仅 FSH 和 FSH 受体，而且 FSH 和 GnRH 受体在小脑皮质的整个浦肯野细胞层、颗粒层和分子层中的一些细胞中共定位。这表明大鼠小脑可以表达 FSH；小脑是 FSH 的靶组织；FSH 可能通过旁分泌或自分泌方式通过 FSH 受体发挥某些功能；GnRH 可能通过小脑中的 GnRH 受体调节 FSH 阳性细胞。我们的研究为进一步研究小脑中 FSH 和相关激素的功能提供了形态学证据。
