Department of Microbiology, Panjab University, Basic Medical Sciences Building, Chandigarh, 160014, India.
Folia Microbiol (Praha). 2010 May;55(3):221-7. doi: 10.1007/s12223-010-0032-7. Epub 2010 Jun 6.
Five bacteriophages (Kpn5, Kpn12, Kpn13, Kpn17 and Kpn22), each having specificity against Klebsiella pneumoniae strain B5055, were isolated from sewage samples and characterized in terms of growth characteristics, genetic material, morphology and structural proteins. Adsorption rate as well as single step growth curve experiments showed variation among phages. Restriction enzyme digestion of DNA confirmed the presence of double stranded DNA as well as the heterogeneous nature of genetic material. RAPD-PCR was performed to further distinguish these closely related phages. Their genome fingerprint confirmed their diversity. Transmission electron microscopy, on the other hand, showed their morphological similarity; they were assigned to family Podoviridae, order Caudovirales on the basis of their head and tail morphology. Structural proteins resolved on SDS-PAGE showed the presence of similar major outer membrane proteins. The bacteriophages, belonging to Podoviridae family with short stumpy tails, were found to be nontoxic to mice. They showed maximum count in various organs at 6 h post inoculation, which persisted till 36 h. These phages thus have the potential to be used for phage therapy.
从污水样本中分离出了 5 种噬菌体（Kpn5、Kpn12、Kpn13、Kpn17 和 Kpn22），每种噬菌体都具有针对肺炎克雷伯菌 B5055 菌株的特异性，并对其生长特性、遗传物质、形态和结构蛋白进行了表征。吸附率和一步生长曲线实验表明噬菌体之间存在差异。DNA 的限制性内切酶消化证实了双链 DNA 的存在以及遗传物质的异质性。RAPD-PCR 进一步用于区分这些密切相关的噬菌体。它们的基因组指纹图谱证实了它们的多样性。透射电子显微镜显示它们的形态相似；根据它们的头部和尾部形态，它们被归类为科尾噬菌体目，目尾噬菌体目。SDS-PAGE 上解析的结构蛋白显示存在类似的主要外膜蛋白。这些噬菌体属于短粗尾巴的尾噬菌体科，对小鼠没有毒性。它们在接种后 6 小时在各种器官中达到最大计数，并持续到 36 小时。因此，这些噬菌体有可能用于噬菌体治疗。
