Li Lin, Liang Shujia, Chen Lili, Liu Wei, Li Hanping, Liu Yongjian, Bao Zuoyi, Wang Zheng, Zhuang Daomin, Liu Siyang, Li Jingyun
Department of AIDS Research, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Fengtai District, Beijing, China.
AIDS Res Hum Retroviruses. 2010 Jun;26(6):699-704. doi: 10.1089/aid.2010.0026.
Guangxi is an important transit area for HIV transmission in South China. Characterization of the full-length genome of HIV-1 prevalent in the area is important for phylogenetic analysis and vaccine development. CRF01_AE is one of the most rapidly spreading subtypes in Guangxi. In this study, we reported thirteen near full-length CRF01_AE genomes from Guangxi, China. The nearly full-length genome was reverse transcripted and amplified in two halves with the 1-kb overlap regions. The PCR products were sequenced directly. The sequence analysis showed that all of 13 strains were CRF01_AE recombinant subtypes. Two clusters were set up with all of the sequences that grouped separately with sequences from Vietnam and Fujian, China, which strongly suggested multiple introductions of CRF01_AE strains into Guangxi province. The results will improve our understanding on the phylogenetic relationship of CRF01_AE strains in South China and also help in the development of a successful HIV vaccine.
广西是中国南方艾滋病病毒传播的重要中转地区。对该地区流行的HIV-1全长基因组进行特征分析，对于系统发育分析和疫苗开发具有重要意义。CRF01_AE是广西传播速度最快的亚型之一。在本研究中，我们报道了来自中国广西的13个近乎全长的CRF01_AE基因组。将近乎全长的基因组进行逆转录，并分成两半进行扩增，重叠区域为1 kb。PCR产物直接进行测序。序列分析表明，所有13株均为CRF01_AE重组亚型。所有序列分为两个簇，分别与来自越南和中国福建的序列分组，这强烈表明CRF01_AE毒株多次传入广西。这些结果将增进我们对中国南方CRF01_AE毒株系统发育关系的理解，也有助于成功开发艾滋病疫苗。
