Department of Microbiology, School of Basic Medicine, Anhui Medical University, Hefei, China.
Department of AIDS Research, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, China.
AIDS Res Hum Retroviruses. 2021 Jul;37(7):572-579. doi: 10.1089/AID.2020.0277. Epub 2021 Jan 25.
Isolation and culture of human immunodeficiency virus (HIV) are an important basis for acquired immune deficiency syndrome (AIDS) etiology, immunology, drug screening, clinical treatment, and vaccine research. CRF01_AE is one of the predominant strains of HIV-1 in China. However, there are few HIV-1 CRF01_AE isolates that have been reported. In this study, 16 HIV-1 CRF01_AE strains from Guangxi, China, were isolated, and the near full-length genomes were reverse transcribed and amplified in two halves with the 1 kb overlapping region. The polymerase chain reaction products were sequenced directly. The phylogenetic analysis results showed that all of the 16 isolated strains were CRF01_AE recombinant form, and two clusters were set up in the phylogenetic tree. The tropic prediction of 16 strains showed that 2 isolates were CCR5 tropic, and the others are CXCR4 tropic. Eight of the isolated strains are drug resistant according to the genetic prediction. These 16 near full-length characterized CRF01_AE isolates obtained in this study will provide valuable genomic and phenotypic information on HIV-1 strains circulating in China for related researches.
分离和培养人类免疫缺陷病毒（HIV）是艾滋病病因学、免疫学、药物筛选、临床治疗和疫苗研究的重要基础。CRF01_AE 是中国 HIV-1 的主要株之一。然而，报道的 HIV-1 CRF01_AE 分离株较少。本研究从中国广西分离出 16 株 HIV-1 CRF01_AE 株，并采用半巢式 PCR 方法以 1kb 重叠区将其全长基因逆转录和扩增为两部分。直接对聚合酶链反应产物进行测序。系统进化分析结果显示，16 株分离株均为 CRF01_AE 重组型，在系统进化树上分为 2 个簇。16 株的嗜性预测显示，2 株为 CCR5 嗜性，其余均为 CXCR4 嗜性。根据遗传预测，8 株分离株具有耐药性。本研究获得的 16 株全长特征 CRF01_AE 分离株将为中国流行的 HIV-1 株提供有价值的基因组和表型信息，用于相关研究。
