Department of Pediatrics, Dr. Peset University Hospital, Valencia, Spain.
Acta Paediatr. 2010 Dec;99(12):1841-6. doi: 10.1111/j.1651-2227.2010.01903.x.
To evaluate the effect of supplementing a hypocaloric diet with mandarin juice, a food with a high content of antioxidants (vitamin C, flavonoids and carotenoids), on biomarkers of oxidant/antioxidant status of severe obese children.
Forty obese children were randomized into two groups pair-wise in a 4-week controlled intervention study. Both groups followed a hypocaloric diet. One group received additionally a supplementation of 500mL of 100% mandarin juice daily. Clinical data, anthropometry, dietary intake and fasting blood samples were collected at baseline and after the intervention. Lipid peroxidation was assessed by circulating levels of malondialdehyde, and protein oxidation was determined by the concentration of plasma carbonyl groups. The antioxidant defence was evaluated by red cell-reduced glutathione and plasma levels of α-tocopherol and vitamin C.
The supplemented group experienced a decrease in the levels of malondialdehyde (-9.6%, p =0.014) and carbonyl groups (-36.1%, p =0.006) and an increase in antioxidants (α-tocopherol +16.1%, p=0.006, glutathione +36.1%, p < 0.0001, and vitamin C + 94.6%, p < 0.0001).
The mandarin juice consumption with a reduced calorie diet positively affects the antioxidant defence and produces a decrease in biomarkers of oxidative stress in obese children.
评估补充富含抗氧化剂（维生素 C、类黄酮和类胡萝卜素）的橘子汁（一种食物）对严重肥胖儿童氧化/抗氧化状态生物标志物的影响。
40 名肥胖儿童在一项为期 4 周的对照干预研究中按 4 周进行配对随机分组。两组均遵循低热量饮食。一组额外补充 500mL 100%橘子汁。在基线和干预后收集临床数据、人体测量学、饮食摄入量和空腹血样。通过循环丙二醛水平评估脂质过氧化，通过血浆羰基浓度测定蛋白质氧化。通过红细胞还原型谷胱甘肽和血浆 α-生育酚和维生素 C 水平评估抗氧化防御能力。
补充组丙二醛（-9.6%，p=0.014）和羰基（-36.1%，p=0.006）水平降低，抗氧化剂（α-生育酚+16.1%，p=0.006，谷胱甘肽+36.1%，p<0.0001，维生素 C+94.6%，p<0.0001）水平升高。
摄入橘子汁与低热量饮食相结合可积极影响抗氧化防御能力，并降低肥胖儿童氧化应激生物标志物水平。
