Codoñer-Franch Pilar, López-Jaén Ana B, Muñiz Pilar, Sentandreu Enrique, Bellés Victoria Valls
Department of Pediatrics, Obstetrics, and Gynecology, University of Valencia, Valencia, Spain.
J Pediatr Gastroenterol Nutr. 2008 Sep;47(3):349-55. doi: 10.1097/MPG.0b013e31816a8cdb.
Oxidative stress has been linked to such degenerative diseases as atherosclerosis, and it has been suggested that increased dietary intake of antioxidants may reduce its progression.
To determine the effect of mandarin juice consumption on biomarkers related to oxidative stress in hypercholesterolemic children.
The diet of 48 children with plasma cholesterol >200 mg/dL and low-density lipoprotein cholesterol >130 mg/dL was supplemented for 28 days with 500 mL/day of pure (100%) mandarin juice (Citrus clementina Hort. ex Tan.). The composition of the mandarin juice was analyzed, and its antioxidant antiradical activity was evaluated in vitro. Malondialdehyde, carbonyl groups, vitamins E and C, erythrocyte-reduced glutathione, and plasma lipids were measured at the onset and at the end of the supplementation period. The paired Student t test was used to compare values before and after supplementation.
Mandarin juice exerted a strong antioxidant effect mainly due to its high hydroxyl activity and, to a lesser extent, to its superoxide scavenger activity. At the end of the study, levels of the plasma biomarkers of oxidative stress were significantly decreased (malondialdehyde -7.4%, carbonyl groups -29.1%, P < 0.01), whereas the plasma antioxidants vitamin E and C (13.5%, P < 0.001 and 68.2%, P < 0.00001, respectively) and intraerythrocyte glutathione level (36.7%, P < 0.00001) were significantly increased. Plasma lipids and antibodies to oxidized low-density lipoproteins remained unchanged.
Regular ingestion of mandarin juice significantly reduces plasma biomarkers of lipid and protein oxidation and enhances the antioxidant status of consumers.
氧化应激与动脉粥样硬化等退行性疾病有关，有人提出增加抗氧化剂的饮食摄入量可能会减缓其发展进程。
确定饮用柑橘汁对高胆固醇血症儿童氧化应激相关生物标志物的影响。
对48名血浆胆固醇>200mg/dL且低密度脂蛋白胆固醇>130mg/dL的儿童的饮食进行为期28天的补充，每天补充500mL纯（100%）柑橘汁（四季橘）。分析了柑橘汁的成分，并在体外评估了其抗氧化抗自由基活性。在补充期开始和结束时测量丙二醛、羰基、维生素E和C、红细胞还原型谷胱甘肽以及血浆脂质。采用配对学生t检验比较补充前后的值。
柑橘汁发挥了强大的抗氧化作用，主要归因于其高羟基活性，其次是其超氧化物清除活性。在研究结束时，氧化应激的血浆生物标志物水平显著降低（丙二醛-7.4%，羰基-29.1%，P<0.01），而血浆抗氧化剂维生素E和C（分别为13.5%，P<0.001和68.2%，P<0.00001）以及红细胞内谷胱甘肽水平（36.7%，P<0.00001）显著升高。血浆脂质和氧化型低密度脂蛋白抗体保持不变。
经常饮用柑橘汁可显著降低脂质和蛋白质氧化的血浆生物标志物，并增强饮用者的抗氧化状态。
