Institute of Bioinformatics and Biotechnology, University of Pune, Pune, 411 007, India.
Biofouling. 2011;27(6):645-54. doi: 10.1080/08927014.2011.594883.
A tropical marine bacterium isolated from the hard coral, Symphyllia sp. was identified as Serratia marcescens on the basis of morphological, biochemical and 16S rDNA analysis. The bacterium showed antimicrobial activity towards the pathogens Candida albicans and Pseudomonas aeruginosa and the marine biofouling bacterium Bacillus pumilus. S. marcescens displayed biosurfactant activity as evidenced by drop collapse, blood hemolysis and surface tension reduction (52.0-27 mN m(-1)). The active compound was purified by solvent extraction and silicic acid chromatography. Characterization was by thin layer chromatography, gas chromatography mass spectroscopy (GC-MS), Fourier transform infrared (FTIR) spectroscopy and (1)H as well as (13)C nuclear magnetic resonance (NMR) analysis. The surfactant was found to be a glycolipid composed of glucose and palmitic acid. The glycolipid prevented adhesion of C. albicans BH, P. aeruginosa PAO1 and B. pumilus TiO1. The glycolipid also disrupted preformed biofilms of these cultures in microtitre plates. Confocal laser scanning microscopy and electron microscopy confirmed the effective removal of biofilms from glass surfaces. The glycolipid derived from S. marcescens could thus serve as a potential anti-biofilm agent.
从硬珊瑚 Symphyllia sp. 中分离到的一株热带海洋细菌，根据形态学、生物化学和 16S rDNA 分析，鉴定为粘质沙雷氏菌。该细菌对病原体白色念珠菌和铜绿假单胞菌以及海洋生物污损细菌解淀粉芽孢杆菌具有抗菌活性。粘质沙雷氏菌表现出生物表面活性剂活性，表现为液滴塌陷、血液溶血和表面张力降低（52.0-27 mN m(-1)）。活性化合物通过溶剂萃取和硅胶色谱法进行纯化。通过薄层层析、气相色谱质谱联用 (GC-MS)、傅里叶变换红外 (FTIR) 光谱和 (1)H 以及 (13)C 核磁共振 (NMR) 分析进行表征。该表面活性剂是由葡萄糖和棕榈酸组成的糖脂。该糖脂可防止白色念珠菌 BH、铜绿假单胞菌 PAO1 和解淀粉芽孢杆菌 TiO1 的黏附。该糖脂还可破坏这些培养物在微量滴定板中形成的预成生物膜。共聚焦激光扫描显微镜和电子显微镜证实可有效去除玻璃表面的生物膜。因此，粘质沙雷氏菌衍生的糖脂可以作为一种潜在的抗生物膜剂。
