Department of General Surgery, the Second Military Medical University affiliated Changhai hospital, Shanghai 200433, China.
J Exp Clin Cancer Res. 2010 Jun 8;29(1):66. doi: 10.1186/1756-9966-29-66.
The prognosis of gastric cancer patients is difficult to predict because of defects in establishing the surgical-pathological features. Cancer-associated fibroblasts (CAFs) have been found to play prominent role in promoting tumor growth, invasion and metastasis. Thus raises the hypothesis that the extent of CAFs prevalence may help to establish the prognosis of gastric cancer patients.
Immunochemistry and realtime-PCR experiments were carried out to compare the expression of proteins which are specific markers of CAFs or secreted by CAFs in the tumor and normal tissue specimens. The extent of CAFs' prevalence was graded according to immunochemical staining, and correlation was further analyzed between CAFs' prevalence and other tumor characteristics which may influence the prognosis of gastric cancer patients.
Nearly 80 percent of normal gastric tissues were negative or weak positive for CAFs staining, while more than 60 percent of gastric cancer tissues were moderate or strong positive for CAFs staining. Realtime-PCR results also showed significant elevated expression of FAP, SDF-1 and TGF-beta1 in gastric cancer tissues compared to normal gastric tissues. Further analysis showed that CAFs' prevalence was correlated with tumor size, depth of the tumor, lymph node metastasis, liver metastasis or peritoneum metastasis.
Reactive cancer associated fibroblasts (CAFs) were frequently accumulated in gastric cancer tissues, and the prevalence of CAFs was correlated with tumor size, depth of the tumor and tumor metastasis, thus give some supports for establishing the prognosis of the gastric cancer patients.
由于在建立手术病理特征方面存在缺陷，胃癌患者的预后难以预测。已经发现癌相关成纤维细胞（CAF）在促进肿瘤生长、侵袭和转移方面发挥着重要作用。因此，提出了这样的假设，即 CAF 流行程度可能有助于确定胃癌患者的预后。
通过免疫化学和实时 PCR 实验比较了肿瘤和正常组织标本中特定 CAF 标志物蛋白或由 CAF 分泌的蛋白的表达。根据免疫化学染色对 CAF 流行程度进行分级，并进一步分析 CAF 流行程度与其他可能影响胃癌患者预后的肿瘤特征之间的相关性。
近 80%的正常胃组织对 CAF 染色呈阴性或弱阳性，而超过 60%的胃癌组织对 CAF 染色呈中度或强阳性。实时 PCR 结果还显示，与正常胃组织相比，胃癌组织中 FAP、SDF-1 和 TGF-β1 的表达显著升高。进一步分析表明，CAF 流行程度与肿瘤大小、肿瘤深度、淋巴结转移、肝转移或腹膜转移有关。
反应性癌相关成纤维细胞（CAF）在胃癌组织中经常聚集，CAF 的流行程度与肿瘤大小、肿瘤深度和肿瘤转移有关，因此为确定胃癌患者的预后提供了一些依据。
