Department of Diagnostic Pathology, Graduate School of Medicine and Pharmaceutical Science, University of Toyama, Toyama, Japan.
Anticancer Res. 2010 Apr;30(4):1233-8.
The oral cavity and pharyngolarynx is readily open to the environment, which provides a good atmosphere to viral infection and subsequently links to the local carcinogenesis. The aim of this study is to clarify the viral risk factors for tongue and pharyngolaryngeal squamous carcinomas and the oncogenic role of DNA viruses.
Tongue, pharyngeal and laryngeal carcinomas, and corresponding non-neoplastic mucosa (NNM) were collected and subjected to microdissection and DNA extraction with integrity detected by beta-globin polymerase chain reaction(PCR). Additionally, we examined genomic DNA copies of Epstein-Barr virus (EBV), human papilloma virus (HPV) 16 and 18, and John Cunningham virus (JCV) by real-time PCR with a comparison of the clinicopathological features of the tumors.
All the extracted DNA samples showed good integrity. Compared with NNM, EBV and HPV16 copies were higher in the three kinds of head and neck carcinoma respectively (p<0.05). The same situation was also observed in tongue and pharyngeal carcinoma for HPV18, and pharyngeal carcinoma for JCV (p<0.05). There were fewer EBV copies in tongue than pharyngeal and laryngeal carcinoma (p<0.05). Pharyngeal carcinoma had a higher HPV16 copy number than tongue and laryngeal carcinoma (p<0.05). Moderately differentiated carcinoma of the head and neck had more EBV copies than well-differentiated (p<0.05).
The viruses studied here might play an important role in the carcinogenesis of tongue and pharyngolaryngeal squamous carcinomas.
口腔和咽喉容易受到环境中病毒的感染，这为病毒感染提供了良好的条件，并与局部致癌作用有关。本研究旨在阐明舌和咽喉鳞癌的病毒危险因素以及 DNA 病毒的致癌作用。
收集舌、咽和喉癌及相应的非肿瘤黏膜（NNM），进行显微解剖和 DNA 提取，并通过β-球蛋白聚合酶链反应（PCR）检测其完整性。此外，我们通过实时 PCR 检测了 Epstein-Barr 病毒（EBV）、人乳头瘤病毒（HPV）16 和 18 以及 John Cunningham 病毒（JCV）的基因组 DNA 拷贝数，并比较了肿瘤的临床病理特征。
所有提取的 DNA 样本均显示出良好的完整性。与 NNM 相比，三种头颈部癌中 EBV 和 HPV16 的拷贝数分别更高（p<0.05）。在舌癌和咽癌中也观察到 HPV18 的相同情况，在咽癌中观察到 JCV 的相同情况（p<0.05）。舌癌中的 EBV 拷贝数少于咽癌和喉癌（p<0.05）。咽癌中的 HPV16 拷贝数高于舌癌和喉癌（p<0.05）。头颈部中分化癌的 EBV 拷贝数高于高分化癌（p<0.05）。
本研究中研究的病毒可能在舌和咽喉鳞状细胞癌的发生中起重要作用。
